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Invitrogen
LAP2 Polyclonal Antibody
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FIGURE: 1 / 15
LAP2 Antibody (PA5-52519) in ICC/IF
Immunofluorescence analysis of LAP2 was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with LAP2 Polyclonal Antibody (Product # PA5-52519) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1... View More
Product Details
PA5-52519
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human LAP2. Recombinant protein control fragment (Product #RP-90160).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: VNPGPIVGTT RKLYEKKLLK LREQGTESRS STPLPTISSS AENTRQNGSN DSDRYSDNEE DSKIELKLEK REPLKGRAKT PVTLKQRRVE HNQSYSQAGI TETEWTSGSS KGGPLQALTR ESTRGSRRTP RKRVETSE
Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 93%.
Target Information
In eukaryotic cells, the nuclear envelope consists of inner and outer membranes which define the border between the nucleus and cytoplasm. The inner and outer nuclear membranes are joined by large, supramolecular assemblies called nuclear pore complexes through which various molecules pass into and out of the nucleus. The inner membrane is lined by a filamentous meshwork called the nuclear lamina made up of proteins called lamins. The attachment of the lamins to the inner nuclear membrane appears to occur through their association with 4 related integral membrane proteins called Lamin Associated Polypeptides (LAPs). LAP 1A, 1B and 1C share a high degree of sequence homology while LAP 2 is distinct from the others. LAP 1A/B bind lamins A, C and B1, while LAP 2 binds lamin B1 only. In addition to lamin binding, LAP 2 has been shown to bind to chromosomes. Phosphorylation of LAP 2 during mitosis inhibits binding to both lamins and chromosomes suggesting that LAP 2 plays an important role in nuclear envelope re-assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: lamina-associated polypeptide 2; Lamina-associated polypeptide 2, isoform alpha; Lamina-associated polypeptide 2, isoform beta; Lamina-associated polypeptide 2, isoforms alpha/zeta; Lamina-associated polypeptide 2, isoforms beta/delta/epsilon/gamma; Lamina-associated polypeptide 2, isoforms beta/gamma; LEM domain containing 4; Thymopoietin (lamina associated polypeptide 2); thymopoietin beta; Thymopoietin isoform alpha; Thymopoietin isoform beta; Thymopoietin isoforms alpha/zeta; Thymopoietin isoforms beta/delta/epsilon/gamma; Thymopoietin, isoforms beta/gamma; Thymopoietin-related peptide isoform alpha; Thymopoietin-related peptide isoforms beta/gamma; TP alpha; TP alpha/zeta; TP beta; TP beta/delta/epsilon/gamma; TP beta/gamma; TPRP isoform alpha; TPRP isoforms beta/gamma
Gene Aliases: 5630400D24Rik; AI195756; AI606875; AW214352; AW547477; CMD1T; LAP2; LEMD4; PRO0868; TMPO; TP
UniProt ID: (Human) P42166, (Mouse) Q61033, (Rat) Q62733
Entrez Gene ID: (Human) 7112, (Mouse) 21917, (Rat) 25359
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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