Invitrogen

LAP3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (8) LAP3 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

LAP3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of LAP3 in human cell line U-251 MG using a LAP3 Polyclonal Antibody (Product # PA5-56206) shows localization to nucleoplasm, cytosol and midbody.

LAP3 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of LAP3 in human kidney, lymph node, skeletal muscle and testis using LAP3 Polyclonal Antibody (Product # PA5-56206) (A) shows similar protein distribution across tissues to an independent LAP3 Polyclonal Antibody (B).

LAP3 Antibody in Western Blot (WB) — Western blot analysis of LAP3 using LAP3 Polyclonal Antibody (Product # PA5-56206) (A) shows similar pattern to an independent LAP3 Polyclonal Antibody (B).

LAP3 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of LAP3 demonstrates antibody specificity. Immunohistochemical analysis of LAP3 using anti-LAP3 Polyclonal Antibody (Product # PA5-56206), shows significant staining of LAP3 in human lymph node and shows minimal or weak staining in human skeletal muscle tissues. The relative expression levels of LAP3 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-56206

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:200-1:500	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human LAP3 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.05 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2643275

Product Specific Information

Immunogen sequence: AGRVVVRRLA VRRFGSRSLS TADMTKGLVL GIYSKEKEDD VPQFTSAGEN FDKLLAGKLR ETLNISGPPL KAGKTRTFYG LHQDFPSVVL VG

Highest antigen sequence identity to the following orthologs: Mouse - 79%, Rat - 82%.

Target Information

Leucine Aminopeptidase 3 (LAP3) is involved in the processing and regular turnover of intracellular proteins. It catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. Release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but reated on arylamides are exceedingly low.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: Cysteinylglycine-S-conjugate dipeptidase; Cytosol aminopeptidase; epididymis secretory protein Li 106; LAP-3; Leucine aminopeptidase 3; Leucyl aminopeptidase; Peptidase S; Proline aminopeptidase; Prolyl aminopeptidase

Gene Aliases: HEL-S-106; LAP; LAP3; LAPEP; PEPS

UniProt ID: (Human) P28838

Entrez Gene ID: (Human) 51056

Molecular Function: metalloprotease