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LARG Polyclonal Antibody

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LARG Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using ARHGEF12 antibody 22441-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

LARG Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis slide using 22441-1-AP ( LARG Antibody) at dilution of 1:50.

LARG Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 22441-1-AP (LARG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Product Details

22441-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LARG Fusion Protein Ag18211 (214-563 aa encoded by BC063117) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.49 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: QRLLKEIQE AKKHIPQLQE QLSKATGSAQ DGAVVTPSRP LGDTLTVSEA ETDPGDVLGR TDCSSGDASR PSSDNADSPK SGPKERIYLE ENPEKSETIQ DTDTQSLVGS PSTRIAPHII GAEDDDFGTE HEQINGQCSC FQSIELLKSR PAHLAVFLHH VVSQFDPATL LCYLYSDLYK HTNSKETRRI FLEFHQFFLD RSAHLKVSVP DEMSADLEKR RPELIPEDLH RHYIQTMQER VHPEVQRHLE DFRQKRSMGL TLAESELTKL DAERDKDRLT LEKERTCAEQ IVAKIEEVLM TAQAVEEDKS STMQYVILMY MKHLGVKVKE PRNLEHKRGR IGFLPKIKQS M (214-563 aa encoded by BC063117)

Target Information

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RSK2 drives cell motility by serine phosphorylation of LARG and activation of Rho GTPases.

Proceedings of the National Academy of Sciences of the United States of America

Shi GX,Yang WS,Jin L,Matter ML,Ramos JW

Tue Jan 09 00:00:00 EST 2018

Bioinformatics

Protein Aliases: leukemia-associated rho guanine nucleotide exchange factor; Leukemia-associated RhoGEF; Rho guanine nucleotide exchange factor (GEF) 12; Rho guanine nucleotide exchange factor 12

Gene Aliases: ARHGEF12; KIAA0382; LARG; PRO2792

UniProt ID: (Human) Q9NZN5

Entrez Gene ID: (Human) 23365

Molecular Function: guanyl-nucleotide exchange factor

Function(s)

G-protein coupled receptor signaling pathway regulation of Rho protein signal transduction intracellular signal transduction positive regulation of apoptotic process positive regulation of GTPase activity regulation of small GTPase mediated signal transduction

Process(es)

G-protein coupled receptor binding guanyl-nucleotide exchange factor activity Rho guanyl-nucleotide exchange factor activity GTPase activator activity protein binding

LARG Polyclonal Antibody

Product Details

22441-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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