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LASP1 Polyclonal Antibody

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LASP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using LASP1 antibody 10515-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

LASP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10515-1-AP (LASP1 antibody) at dilution of 1:50 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

LASP1 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10515-1-AP (LASP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

LASP1 Antibody in Immunoprecipitation (IP) — IP result of anti-LASP1 (IP: 10515-1-AP, 4 µg; Detection: 10515-1-AP 1:1500) with rat brain tissue lysate 4000 µg.

Product Details

10515-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 8 publications 8 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-
Immunohistochemistry (IHC)	-	View 4 publications 4 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LASP1 Fusion Protein Ag0800 (125-210 aa encoded by BC012460) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.44 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: EFEKSRMGP SGGEGMEPER RDSQDGSSYR RPLEQQQPHH IPTSAPVYQQ PQQQPVAQSY GGYKEPAAPV SIQRSAPGGG GKRYRAA (125-210 aa encoded by BC012460 )

Target Information

This gene encodes a member of a LIM protein subfamily characterized by a LIM motif and a domain of Src homology region 3. The encoded protein functions as an actin-binding protein and possibly in cytoskeletal organization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LASP1 promotes glioma cell proliferation and migration and is negatively regulated by miR-377-3p.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Liu Y,Gao Y,Li D,He L,Iw L,Hao B,Chen X,Cao Y

Sat Dec 01 00:00:00 EST 2018

LASP1 promotes glioma cell proliferation and migration and is negatively regulated by miR-377-3p.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Liu Y,Gao Y,Li D,He L,Iw L,Hao B,Chen X,Cao Y

Sat Dec 01 00:00:00 EST 2018

Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.

Journal of experimental & clinical cancer research : CR

Lian Y,Xiong F,Yang L,Bo H,Gong Z,Wang Y,Wei F,Tang Y,Li X,Liao Q,Wang H,Zhou M,Xiang B,Wu X,Li Y,Li X,Chen X,Li G,Guo C,Zeng Z,Xiong W

Tue Oct 16 00:00:00 EDT 2018

Change in distribution of cytoskeleton-associated proteins, lasp-1 and palladin, during uterine receptivity in the rat endometrium.

Reproduction, fertility, and development

Nicholson L,Lindsay L,Murphy CR

Mon Oct 01 00:00:00 EDT 2018

Change in distribution of cytoskeleton-associated proteins, lasp-1 and palladin, during uterine receptivity in the rat endometrium.

Reproduction, fertility, and development

Nicholson L,Lindsay L,Murphy CR

Mon Oct 01 00:00:00 EDT 2018

Upregulated LASP-1 correlates with a malignant phenotype and its potential therapeutic role in human cholangiocarcinoma.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Zhang H,Li Z,Chu B,Zhang F,Zhang Y,Ke F,Chen Y,Xu Y,Liu S,Zhao S,Liang H,Weng M,Wu X,Li M,Wu W,Quan Z,Liu Y,Zhang Y,Gong W

Wed Jun 01 00:00:00 EDT 2016

Upregulated LASP-1 correlates with a malignant phenotype and its potential therapeutic role in human cholangiocarcinoma.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Zhang H,Li Z,Chu B,Zhang F,Zhang Y,Ke F,Chen Y,Xu Y,Liu S,Zhao S,Liang H,Weng M,Wu X,Li M,Wu W,Quan Z,Liu Y,Zhang Y,Gong W

Wed Jun 01 00:00:00 EDT 2016

LASP-1 induces proliferation, metastasis and cell cycle arrest at the G2/M phase in gallbladder cancer by down-regulating S100P via the PI3K/AKT pathway.

Cancer letters

Li Z,Chen Y,Wang X,Zhang H,Zhang Y,Gao Y,Weng M,Wang L,Liang H,Li M,Zhang F,Zhao S,Liu S,Cao Y,Shu Y,Bao R,Zhou J,Liu X,Yan Y,Zhen L,Dong Q,Liu Y

Mon Mar 28 00:00:00 EDT 2016

LASP-1 induces proliferation, metastasis and cell cycle arrest at the G2/M phase in gallbladder cancer by down-regulating S100P via the PI3K/AKT pathway.

Cancer letters

Li Z,Chen Y,Wang X,Zhang H,Zhang Y,Gao Y,Weng M,Wang L,Liang H,Li M,Zhang F,Zhao S,Liu S,Cao Y,Shu Y,Bao R,Zhou J,Liu X,Yan Y,Zhen L,Dong Q,Liu Y

Mon Mar 28 00:00:00 EDT 2016

The tumor-suppressive function of miR-1 by targeting LASP1 and TAGLN2 in esophageal squamous cell carcinoma.

Journal of gastroenterology and hepatology

Du YY,Zhao LM,Chen L,Sang MX,Li J,Ma M,Liu JF

Mon Feb 01 00:00:00 EST 2016

The tumor-suppressive function of miR-1 by targeting LASP1 and TAGLN2 in esophageal squamous cell carcinoma.

Journal of gastroenterology and hepatology

Du YY,Zhao LM,Chen L,Sang MX,Li J,Ma M,Liu JF

Mon Feb 01 00:00:00 EST 2016

AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Zeng Z,Bo H,Gong Z,Lian Y,Li X,Li X,Zhang W,Deng H,Zhou M,Peng S,Li G,Xiong W

Fri Jan 01 00:00:00 EST 2016

Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.

Oncotarget

Bo H,Gong Z,Zhang W,Li X,Zeng Y,Liao Q,Chen P,Shi L,Lian Y,Jing Y,Tang K,Li Z,Zhou Y,Zhou M,Xiang B,Li X,Yang J,Xiong W,Li G,Zeng Z

Fri Aug 21 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: LASP-1; lasp1 {ECO:0000312|RGD:68408}; LIM and SH3 domain protein 1; Metastatic lymph node gene 50 protein; MLN 50; Tg(11Col1a1)NG

Gene Aliases: AA408629; Def-4; Lasp-1; LASP1; MLN50; SH3P6; Tg(Col1a1-lacZ)1Ngma

UniProt ID: (Human) Q14847, (Mouse) Q61792, (Rat) Q99MZ8

Entrez Gene ID: (Human) 3927, (Mouse) 16796, (Rat) 29278

Function(s)

ion transport positive regulation of signal transduction ion transmembrane transport cell-cell adhesion transport

Process(es)

SH3/SH2 adaptor activity protein binding zinc ion binding ion transmembrane transporter activity actin filament binding cadherin binding involved in cell-cell adhesion actin binding metal ion binding

LASP1 Polyclonal Antibody

Product Details

10515-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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