Western blot analysis of LASP1 in mouse brain extracts (RIPA buffer, 35g total protein per lane). Samples were probed with a LASP1 polyclonal antibody (Product # PA1-31450) at a dilution of 0.5mg/ml. Samples were incubated for 1 hour and detected using chemiluminescence.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids: NPNCARCGKIVYP (N-Terminus) of human LASP1|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31450 detects LASP1 from mouse, human samples.
PA1-31450 has been successfully used in Western blot applications.
The PA1-31450 immunogen is: Synthetic peptide - amino acids: NPNCARCGKIVYP (N-Terminus) of human LASP1
This gene encodes a member of a LIM protein subfamily characterized by a LIM motif and a domain of Src homology region 3. The encoded protein functions as an actin-binding protein and possibly in cytoskeletal organization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.