|Tested species reactivity||Bacteria, Bovine, Dog, Hamster, Human, Mouse, Non-human primate, Pig, Rat, Zebrafish|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide between amino acids 1-100 of the human LC3 protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:400|
|Immunoprecipitation (IP)||20 µg/500 µg of protein|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The reaction with mouse LC3 is weaker than human.
The immunogen for this antibody production has 100% sequence homology with rat and zebrafish, 92% with mouse, and 91% with bovine proteins.
LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Short communication: Effect of heat stress on markers of autophagy in the mammary gland during the dry period.
PA1-16930 was used in western blot to study the mammary gland during the dry period and the effect of heat stress on markers of autophagy
|Wohlgemuth SE,Ramirez-Lee Y,Tao S,Monteiro AP,Ahmed BM,Dahl GE||Journal of dairy science (99:4875)||2016|
Mitophagy promotes replication of oncolytic Newcastle disease virus by blocking intrinsic apoptosis in lung cancer cells.
PA1-16930 was used in western blot to study the promotion of mitophagy by oncolytic Newcastle disease virus on and its effect on viral replication
|Meng G,Xia M,Wang D,Chen A,Wang Y,Wang H,Yu D,Wei J||Oncotarget (5:6365)||2014|
Mitophagy enhances oncolytic measles virus replication by mitigating DDX58/RIG-I-like receptor signaling.
PA1-16930 was used in western blot to study the mechanism for the oncolytic measles virus replication
|Xia M,Gonzalez P,Li C,Meng G,Jiang A,Wang H,Gao Q,Debatin KM,Beltinger C,Wei J||Journal of virology (88:5152)||2014|
Vitamin E as a novel enhancer of macroautophagy in rat hepatocytes and H4-II-E cells.
PA1-16930 was used in western blot to investigate the effect of vitamin E on macroautophagy in liver cells
|Karim MR,Fujimura S,Kadowaki M||Biochemical and biophysical research communications (394:981)||2010|
Reactivation of autophagy by spermidine ameliorates the myopathic defects of collagen VI-null mice.
PA1-16930 was used in western blot to assess the effects of an autophagy inducer on col6a1 knock out mice
|Chrisam M,Pirozzi M,Castagnaro S,Blaauw B,Polishchuck R,Cecconi F,Grumati P,Bonaldo P||Autophagy (11:2142)||2016|