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Invitrogen
LC3A Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
LC3A Antibody (PA5-22990) in ICC/IF
Immunocytochemistry analysis of LC3A in HeLa cells. Samples were incubated in LC3A polyclonal antibody (Product # PA5-22990) followed by DyLight 488 (green). Cells were treated overnight with 50 µM chloroquine to induce autophagosome formation. Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Product Details
PA5-22990
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat,
Zebrafish
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Genomic sequence made to a N-terminal portion of the human LC3A protein.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11154460
Product Specific Information
In ICC/IF, autophagosome formation has been seen in HeLa cells after treatment with 50uM chloroquine.
Suggested positive control: human brain lysate, LC3 overexpression lysate.
Target Information
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; Microtubule-asso; Microtubule-associated protein 1 light chain 3 alpha; microtubule-associated protein 1-light chain 3A; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3A; MLP3A; RP11-346K17.1
Gene Aliases: 1010001H21Rik; 4922501H04Rik; ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3; MAP1LC3A; zgc:77094
UniProt ID: (Human) Q9H492, (Bovine) Q2HJ23, (Rat) Q6XVN8, (Mouse) Q91VR7
Entrez Gene ID: (Human) 84557, (Bovine) 514547, (Rat) 362245, (Mouse) 66734, (Zebrafish) 406466
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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