Invitrogen

LC3A/LC3B Polyclonal Antibody

Product Image Gallery

LC3A/LC3B Antibody — Figure 5 Effects of Drp1 inhibitor Mdivi-1 on mitochondrial autophagy, metabolism, and viability of MDA-MB-231 cancer cells. (a)-(b) Mdivi-1 suppressed mitophagy marker BNIP3 and caused accumulation of LC3-II, indicative of reduced autophagic flux. MDA-MB-231 cells were cultured and treated with Mdivi-1 as described in the Materials and Methods, and the protein levels were analyzed by western blot (a) and densitometric (b) analysis; n = 3-5. (c)-(d) Mdivi-1 promoted mitochondrial oxidative capacity, as evaluated by respiratory control ratios (RCR) and ATP turnover. (e) Mdivi-1 suppressed glycolysis ( n = 3). (f) Mdivi-1 reduced breast cancer cell viability ( n = 5-7). ** p < 0.01 and *** p < 0.001.

LC3A/LC3B Antibody in Immunofluorescence (IF) — Immunocytochemistry/Immunofluorescence analysis of LC3 in HeLa cells using an LC3A/LC3B polyclonal antibody (Product # PA1-16931) with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

LC3A/LC3B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical (paraffin) staining of Human ovarian Cancer tissue stained using heat mediated antigen retrieval in pH 6.0 citrate buffer and an LC3A/LC3B polyclonal antibody (Product # PA1-16931) at 1:200 dilution.

LC3A/LC3B Antibody in Immunohistochemistry (IHC) — Immunohistochemistry staining of human brain, cerebral cortex, neurons with cell processes using an LC3A/LC3B polyclonal antibody (Product # PA1-16931).

LC3A/LC3B Antibody in Western Blot (WB) — Western blot analysis of LC3 in mouse ES cell lysates (Atg5-/- ES cells) using an LC3A/LC3B polyclonal antibody (Product # PA1-16931).

Product Details

Tested Applications

Dilution

ELISA (ELISA)

Assay-Dependent

Flow Cytometry (Flow)

Assay-Dependent

Immunocytochemistry (ICC)

1:100-1:300

Immunofluorescence (IF)

1:100-1:300

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

Immunoprecipitation (IP)

20 µg/500 µg of lysate

Western Blot (WB)

2 µg/mL

Published Applications

Western Blot (WB)

See 7 publications below

Immunofluorescence (IF)

See 1 publication below

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat, Zebrafish

Published species

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region between residues 25-121 of human LC3 protein.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

RRID

AB_2137583

Product Specific Information

This antibody detects both LC3A and LC3B.

In Western blot bands are seen ~17 and 19 kDa.

Suggested positive control: human and mouse brain lysate, antigen standard for MAP1LC3B (transient overexpression lysate), mouse brain protein, human brain protein.

Target Information

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation.

During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment.

There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associate with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Autophagy-related protein LC3 A; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 A; Autophagy-related ubiquitin-like modifier LC3 B; LC3; MAP1 light chain 3-like protein 1; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 A; MAP1A/1B light chain 3 B; MAP1A/MAP1B LC3; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B light chain 3 B; MAP1LC3; MAP1LC3A; MAP1LC3B; Microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1-light chain 3A; Microtubule-associated proteins 1A/1B light chain; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3A; Microtubule-associated proteins 1A/1B light chain 3B

Gene Aliases: 1010001C15Rik; 1010001H21Rik; 4922501H04Rik; Atg8; ATG8E; ATG8F; LC3; LC3A; LC3B; MAP1A/1BLC3; MAP1ALC3; MAP1BLC3; Map1lc3; MAP1LC3A; MAP1LC3B; MAP1LC3B-a; Mpl3; wu:fb60g11; zbs559; zgc:56434; zgc:77094

UniProt ID: (Human) Q9H492, (Human) Q9GZQ8, (Rat) Q6XVN8, (Mouse) Q91VR7, (Rat) Q62625, (Mouse) Q9CQV6

Entrez Gene ID: (Human) 84557, (Dog) 477201, (Human) 81631, (Dog) 479619, (Rat) 362245, (Mouse) 66734, (Rat) 64862, (Mouse) 67443, (Zebrafish) 406466, (Zebrafish) 322425