|Tested species reactivity||Dog, Human, Mouse, Rat, Zebrafish|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region between residues 25-121 of human LC3 protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:400|
|Immunoprecipitation (IP)||20 µg/500 µg of lysate|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 5 publications below|
This antibody detects both LC3A and LC3B.
In Western blot bands are seen ~17 and 19 kDa.
Suggested positive control: human and mouse brain lysate, antigen standard for MAP1LC3B (transient overexpression lysate), mouse brain protein, human brain protein.
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation.
During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment.
There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associate with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Amelioration of neuronal cell death in a spontaneous obese rat model by dietary restriction through modulation of ubiquitin proteasome system.
PA1-16931 was used in western blot to characterize amelioration of neuronal cell death in a rat model of spontaneous obesity that is dietary-restricted through modulation of ubiquitin proteasome system
|Shruthi K,Reddy SS,Reddy PY,Shivalingam P,Harishankar N,Reddy GB||The Journal of nutritional biochemistry (33:73)||2016|
MicroRNA-34a Modulates Neural Stem Cell Differentiation by Regulating Expression of Synaptic and Autophagic Proteins.
PA1-16931 was used in western blot to identify Syt1 and Atg9a as targets of miR-34a during neuronal stem cell differentiation
|Morgado AL,Xavier JM,Dionísio PA,Ribeiro MF,Dias RB,Sebastião AM,Solá S,Rodrigues CM||Molecular neurobiology (51:1168)||2015|
K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy.
PA1-16931 was used in western blot to report that K63 ubiquitination is required for chaperone-mediated autophagy degradation of HIF1A in vitro and in vivo.
|Ferreira JV,Soares AR,Ramalho JS,Pereira P,Girao H||Scientific reports (5:null)||2015|
Mitochondrial translocation of p53 modulates neuronal fate by preventing differentiation-induced mitochondrial stress.
PA1-16931 was used in immunocytochemistry and western blot to study the role of p53 mitochondrial translocation in modulating mitochondrial stress induced by differentiation and the significance for neuronal cell fate
|Xavier JM,Morgado AL,Solá S,Rodrigues CM||Antioxidants and redox signaling (21:1009)||2014|
Amyloid ß peptides promote autophagy-dependent differentiation of mouse neural stem cells: Aß-mediated neural differentiation.
PA1-16931 was used in western blot to study the ability of different beta-amyloid peptides to drive murine neural stem cell differentiation and the role played by autophagy
|Fonseca MB,Solá S,Xavier JM,Dionísio PA,Rodrigues CM||Molecular neurobiology (48:829)||2013|