Invitrogen

LC3A/LC3B Polyclonal Antibody

Product Image Gallery

LC3A/LC3B Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of LC3 in untreated HeLa cells (left panel) and HeLa cells treated with 50µM CQ overnight (right panel). Cells were fixed for 10 min using 10% formalin and permeabilized for 5 min using 1X PBS with 0.05% Triton X-100. Cells were incubated with anti-LC3 polyclonal antibody (Product # PA1-16931) at 5 µg/mL overnight at 4ºC, followed by detection using an anti-mouse DyLight 488 (Green) at a dilution of 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X magnification.

LC3A/LC3B Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of LC3 in human cerebral cortex tissue using anti-LC3 polyclonal antibody (Product # PA1-16931).

LC3A/LC3B Antibody in Western Blot (WB) — Western blot analysis of mouse heart tissue lysates subjected to 48 hours of starvation compared to non-starved lysates using anti-LC3 polyclonal antibody (Product # PA1-16931). Results show higher levels of LC3-II in the heart tissue lysates from starved mice.

LC3A/LC3B Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Published figure using LC3A/LC3B polyclonal antibody (Product # PA1-16931) in Western Blot and Immunocytochemistry

Product Details

PA1-16931

Applications	Tested Dilution	Publications
Western Blot (WB)	1:50	View 11 publications 11 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:400	-
Immunohistochemistry (Frozen) (IHC (F))	Assay-Dependent	-
Immunocytochemistry (ICC/IF)	1:100-1:300	View 4 publications 4 publications
Immunocytochemistry (ICC)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	Assay-Dependent	-
ELISA (ELISA)	Assay-Dependent	-
Immunoprecipitation (IP)	20 µg/500 µg of lysate	-

Product Specifications
Species Reactivity	Dog, Fish, Human, Mouse, Plant, Rat, Zebrafish
Published species	Fish, Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to a region between residues 25-121 of human LC3 protein.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	-20°C or -80°C if preferred
RRID	AB_2137583

Product Specific Information

This antibody detects both LC3A and LC3B.|Western blot bands are seen at ~19 kDa, representing LC3-I, and ~17 kDa, representing LC3-II. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Recommended positive controls: LC3B overexpression lysate, mouse brain whole tissue lysate (adult whole normal), human brain whole tissue lysate (adult whole normal), Neuro2a whole cell lysate, Neuro2a Chloroquine-treated/untreated cell lysate, HeLa chloroquine-treated/untreated cell lysate

This antibody is predicted to react with Bovine based on 100% sequence homology.

Target Information

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of Calcineurin/NFAT Signaling Blocks Oncogenic H-Ras Induced Autophagy in Primary Human Keratinocytes.

Frontiers in cell and developmental biology

Wang S,Qian H,Zhang L,Liu P,Zhuang D,Zhang Q,Bai F,Wang Z,Yan Y,Guo J,Huang J,Wu X

Published figure using LC3A/LC3B polyclonal antibody (Product # PA1-16931) in Western Blot

Fri Aug 06 00:00:00 EDT 2021

Early Intracellular Trafficking and Subsequent Activity of Programmed Cell Death in Channel Catfish Macrophages Infected with Edwardsiella ictaluri.

Microorganisms

Dubytska LP,Thune RL

PA1-16931 was used in Immunocytochemistry to study trafficking and programmed cell death in Edwardsiella ictaluri-infected catfish macrophages.

Sat Oct 24 00:00:00 EDT 2020

Early Intracellular Trafficking and Subsequent Activity of Programmed Cell Death in Channel Catfish Macrophages Infected with Edwardsiella ictaluri.

Microorganisms

Dubytska LP,Thune RL

PA1-16931 was used in Immunocytochemistry to study trafficking and programmed cell death in Edwardsiella ictaluri-infected catfish macrophages.

Sat Oct 24 00:00:00 EDT 2020

Sinoporphyrin sodium is a promising sensitizer for photodynamic and sonodynamic therapy in glioma.

Oncology reports

An YW,Liu HQ,Zhou ZQ,Wang JC,Jiang GY,Li ZW,Wang F,Jin HT

PA1-16931 was used in Western Blotting to explore the antitumor effects of sinoporphyrin sodium (DVDMS)‚mediated photodynamic therapy (PDT) and sonodynamic therapy (SDT) in glioma, and to reveal the underlying mechanisms.

Thu Oct 01 00:00:00 EDT 2020

Autophagy activation in circulating proangiogenic cells aggravates AKI in type I diabetes mellitus.

American journal of physiology. Renal physiology

Schwarze K,Kribben A,Ritter O,Müller GA,Patschan D

PA1-16931 was used in Western Blotting to utilize two established autophagy inducers for pharmacological preconditioning of so-called proangiogenic cells (PACs) in PAC treatment of diabetic AKI.

Mon Oct 01 00:00:00 EDT 2018

Pazopanib Reduces Phosphorylated Tau Levels and Alters Astrocytes in a Mouse Model of Tauopathy.

Journal of Alzheimer's disease : JAD

Javidnia M,Hebron ML,Xin Y,Kinney NG,Moussa CE

Published figure using LC3A/LC3B polyclonal antibody (Product # PA1-16931) in Western Blot

Mon Apr 09 00:00:00 EDT 2018

Autophagy negatively regulates pro-osteogenic activity in human aortic valve interstitial cells.

The Journal of surgical research

Deng XS,Meng X,Venardos N,Song R,Yamanaka K,Fullerton D,Jaggers J

PA1-16931 was used in Immunohistochemistry-immunofluorescence to suggest that upregulation of autophagy may prevent the progression of calcific aortic valve disease.

Sun Oct 01 00:00:00 EDT 2017

Autophagy negatively regulates pro-osteogenic activity in human aortic valve interstitial cells.

The Journal of surgical research

Deng XS,Meng X,Venardos N,Song R,Yamanaka K,Fullerton D,Jaggers J

PA1-16931 was used in Immunohistochemistry-immunofluorescence to suggest that upregulation of autophagy may prevent the progression of calcific aortic valve disease.

Sun Oct 01 00:00:00 EDT 2017

Coordinated Upregulation of Mitochondrial Biogenesis and Autophagy in Breast Cancer Cells: The Role of Dynamin Related Protein-1 and Implication for Breast Cancer Treatment.

Oxidative medicine and cellular longevity

Zou P,Liu L,Zheng LD,Payne KK,Manjili MH,Idowu MO,Zhang J,Schmelz EM,Cheng Z

PA1-16931 was used in western blot to study mitochondrial biogenesis and mitophagy in cancer cells

Mon Mar 13 00:00:00 EDT 2017

Amelioration of neuronal cell death in a spontaneous obese rat model by dietary restriction through modulation of ubiquitin proteasome system.

The Journal of nutritional biochemistry

Shruthi K,Reddy SS,Reddy PY,Shivalingam P,Harishankar N,Reddy GB

PA1-16931 was used in western blot to characterize amelioration of neuronal cell death in a rat model of spontaneous obesity that is dietary-restricted through modulation of ubiquitin proteasome system

Fri Jul 01 00:00:00 EDT 2016

MEK5/ERK5 signaling inhibition increases colon cancer cell sensitivity to 5-fluorouracil through a p53-dependent mechanism.

Oncotarget

Pereira DM,Simões AE,Gomes SE,Castro RE,Carvalho T,Rodrigues CM,Borralho PM

PA116931 was used in western blot to assess the contribution of MEK5/ERK5 signaling in the sensitivity of colon cancer cells to 5-fluorouracil

Tue Jun 07 00:00:00 EDT 2016

MicroRNA-34a Modulates Neural Stem Cell Differentiation by Regulating Expression of Synaptic and Autophagic Proteins.

Molecular neurobiology

Morgado AL,Xavier JM,Dionísio PA,Ribeiro MF,Dias RB,Sebastião AM,Solá S,Rodrigues CM

PA1-16931 was used in western blot to identify Syt1 and Atg9a as targets of miR-34a during neuronal stem cell differentiation

Fri Feb 12 00:00:00 EST 2016

K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy.

Scientific reports

Ferreira JV,Soares AR,Ramalho JS,Pereira P,Girao H

PA1-16931 was used in western blot to report that K63 ubiquitination is required for chaperone-mediated autophagy degradation of HIF1A in vitro and in vivo.

Mon May 11 00:00:00 EDT 2015

Mitochondrial translocation of p53 modulates neuronal fate by preventing differentiation-induced mitochondrial stress.

Antioxidants & redox signaling

Xavier JM,Morgado AL,Solá S,Rodrigues CM

PA1-16931 was used in immunocytochemistry and western blot to study the role of p53 mitochondrial translocation in modulating mitochondrial stress induced by differentiation and the significance for neuronal cell fate

Mon Sep 01 00:00:00 EDT 2014

Mitochondrial translocation of p53 modulates neuronal fate by preventing differentiation-induced mitochondrial stress.

Antioxidants & redox signaling

Xavier JM,Morgado AL,Solá S,Rodrigues CM

Mon Sep 01 00:00:00 EDT 2014

Amyloid β peptides promote autophagy-dependent differentiation of mouse neural stem cells: Aβ-mediated neural differentiation.

Molecular neurobiology

Fonseca MB,Solá S,Xavier JM,Dionísio PA,Rodrigues CM

PA1-16931 was used in western blot to study the ability of different beta-amyloid peptides to drive murine neural stem cell differentiation and the role played by autophagy

Sun Dec 01 00:00:00 EST 2013

STUB1/CHIP is required for HIF1A degradation by chaperone-mediated autophagy.

Autophagy

Ferreira JV,Fôfo H,Bejarano E,Bento CF,Ramalho JS,Girão H,Pereira P

PA1-16931 was used in Immunocytochemistry to show that HIF1A can be targeted for degradation in the lysosome by CMA and this targeting is dependent on its interaction with the ubiquitin ligase STUB1.

Sun Sep 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Autophagy-related protein LC3 A; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 A; Autophagy-related ubiquitin-like modifier LC3 B; Lc3; MAP1 light chain 3-like protein 1; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 A; MAP1A/1B light chain 3 B; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1-light chain 3A; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3A; Microtubule-associated proteins 1A/1B light chain 3B

Gene Aliases: 1010001C15Rik; 1010001H21Rik; 4922501H04Rik; Atg8; ATG8E; ATG8F; LC3; LC3A; LC3B; MAP1A/1BLC3; MAP1ALC3; MAP1BLC3; Map1lc3; MAP1LC3A; MAP1LC3B; MAP1LC3B-a; Mpl3; wu:fb60g11; zbs559; zgc:56434; zgc:77094

UniProt ID: (Human) Q9H492, (Human) Q9GZQ8, (Rat) Q6XVN8, (Mouse) Q91VR7, (Rat) Q62625, (Mouse) Q9CQV6

Entrez Gene ID: (Human) 84557, (Dog) 477201, (Human) 81631, (Dog) 479619, (Rat) 362245, (Mouse) 66734, (Rat) 64862, (Mouse) 67443, (Zebrafish) 406466, (Zebrafish) 322425