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Invitrogen
LC3B Polyclonal Antibody, DyLight™ 488
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FIGURE: 1 / 2
LC3B Antibody (PA5-22938) in WB
Western blot was performed using Anti-LC3B Rabbit Polyclonal Antibody (Product # PA5-22938) and 14-16 kDa bands corresponding to LC3B were observed across cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with Chloroquine (50uM for 12 Hours) (Lane 2), HCT 116 (Lane 3) and HCT 116 treated with Chloroquine (50uM for 12 Hours) (Lane 4) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product #) by iBlot®... View More
Product Details
PA5-22938
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria,
Bovine,
Dog,
Human,
Mouse,
Non-human primate,
Pig,
Rat,
Yeast,
Zebrafish
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide made to the N-terminal region of the human LC3B protein.
Conjugate
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
0.82 mg/mL
Amount
82 µg
Purification
Antigen affinity chromatography
Storage buffer
50mM sodium borate
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans, is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B). LC3B is associated with microtubule assembly and important is in neurogenesis. Recent studies indicate that LC3B plays a vital role in autophagy, a process that involves the bulk degradation of cytoplasmic component. Three human LC3 isoforms undergo post-translational modifications during autophagy. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). LC3B is a microtubule-associated protein that mediate the physical interactions between microtubules and components of the cytoskeleton. LC3B may play a role in processes involving cancer, aging, metabolic and neurodegenerative disorders and cardiovascular/pulmonary diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATG8E; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC3; LC3 beta; LC3A; MAP1 light chain 3-like protein 2; MAP1A/1B; MAP1A/1B LC3; MAP1A/1B light chain 3 B; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B LC3 B-like; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B light chain 3 B-like; MAP1A1B-LC3; MAP1BLC3; MAP1LC3A; Microtubule-associated protein 1 light chain 3 beta; Microtubule-associated protein 1 light chain 3 beta 2; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3 beta 2; Microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated proteins 1A/1B light chain 3B-like; Microtubule-associated proteins 1A/1B light chain 3B-like protein; RP11-346K17.1; unnamed protein product
Gene Aliases: 1010001C15Rik; Atg8; ATG8F; ATG8G; LC3B; MAP1A/1BLC3; MAP1ALC3; MAP1LC3; MAP1LC3B; MAP1LC3B-a; MAP1LC3B2; Mpl3; wu:fb60g11; zbs559; zgc:56434
UniProt ID: (Bovine) O41515, (Human) Q9GZQ8, (Human) A6NCE7, (Rat) Q62625, (Mouse) Q9CQV6
Entrez Gene ID: (Bovine) 408001, (Dog) 479619, (Pig) 100462754, (Human) 81631, (Human) 643246, (Rat) 64862, (Mouse) 67443, (Zebrafish) 322425
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