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Invitrogen
LCAT Polyclonal Antibody
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FIGURE: 1 / 4
LCAT Antibody (PA5-22965) in ICC/IF
Immunocytochemistry analysis of LCAT in HeLa cells. Samples were incubated with LCAT polyclonal antibody (Product # PA5-22965) using a dilution of 1:500 followed by Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).
Product Details
PA5-22965
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carboxyl-terminal histidine-tagged mouse recombinant LCAT purified from the medium of CHO cells overexpressing mouse LCAT cDNA.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive control: antigen standard for LCAT (transient overexpression lysate), Lipoprotein fraction of whole mouse plasma.
Target Information
Lecithin: cholesterol acyltransferase (LCAT) is the plasma enzyme responsible for most HDL cholesteryl esters in human plasma. In species with cholesteryl ester transfer protein (CETP) it is also responsible for a significant proportion of VLDL and LDL cholesteryl esters. LCAT, through the esterification of cholesterol, plays a role in the reverse cholesterol transport pathway by facilitating the net movement of cholesterol from peripheral tissues back to the liver for excretion. LCAT deficiency results in low levels of plasma HDL, whereas a transgenic overexpression of LCAT results in increased plasma HDL concentrations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1-alkyl-2-acetylglycerophosphocholine esterase; Lecithin-cholesterol acyltransferase; lecithin-cholesterol acyltransferase Lcat; PAF acetylhydrolase; phosphatidylcholine--sterol O-acyltransferase; Phosphatidylcholine-sterol acyltransferase; Phospholipid-cholesterol acyltransferase; Platelet-activating factor acetylhydrolase; testicular secretory protein Li 24
Gene Aliases: AI046659; D8Wsu61e; LCAT
UniProt ID: (Human) P04180, (Mouse) P16301
Entrez Gene ID: (Human) 3931, (Mouse) 16816
phospholipid metabolic process
phosphatidylcholine biosynthetic process
cholesterol metabolic process
cholesterol transport
very-low-density lipoprotein particle remodeling
high-density lipoprotein particle remodeling
cholesterol esterification
lipoprotein metabolic process
lipoprotein biosynthetic process
cholesterol homeostasis
reverse cholesterol transport
phosphatidylcholine metabolic process
response to copper ion
response to glucocorticoid
regulation of high-density lipoprotein particle assembly
lipid metabolic process
steroid metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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