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Rockland
LDB1 Polyclonal Antibody
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FIGURE: 1 / 1
LDB1 Antibody (600-401-350) in WB
Western blot using Rocklands affinity purified anti-LDB1 antibody shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 µg of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:20,000 dilution of DyLight™800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-145-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made ... View More
Product Details
600-401-350
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of mouse LDB1 protein.
Conjugate
Form
Liquid
Concentration
1.8 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against the mouse LDB1protein. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, frog, chicken and rat based on 100% homology for the immunogen sequence. Cross reactivity with LDB1 homologues from other sources has not been determined.
Target Information
LIM domain-binding protein 1 (LDB1), also known as Carboxyl-terminal LIM domain-binding protein 2 (CLIM2), binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. LDB1 may regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. It plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. LDB1 acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. It also acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state. LDB1 strongly interacts with the LIM2 domain of LMX1A to form homodimer. LDB1 protein is expressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Due to alternative splicing events, three isoforms exist for LDB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: carboxy terminal LIM domain protein 2; Carboxyl-terminal LIM domain-binding protein 2; CLIM-2; hLdb1; LDB-1; LIM domain-binding factor CLIM2; LIM domain-binding factor-1; LIM domain-binding protein 1; mLdb1; Nuclear LIM interactor; unnamed protein product
Gene Aliases: CLIM-2; CLIM2; LDB-1; LDB1; NLI
UniProt ID: (Human) Q86U70, (Mouse) P70662
Entrez Gene ID: (Human) 8861, (Rat) 309447, (Mouse) 16825
DNA binding
chromatin binding
transcription cofactor activity
transcription coactivator activity
protein binding
enzyme binding
LIM domain binding
identical protein binding
protein homodimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
DNA-binding transcription factor binding
transcription factor activity, transcription factor binding
RNA polymerase II activating transcription factor binding
enhancer sequence-specific DNA binding
protein self-association
transcription cofactor
negative regulation of transcription from RNA polymerase II promoter
transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery
gastrulation with mouth forming second
hair follicle development
transcription from RNA polymerase II promoter
cell adhesion
nervous system development
anterior/posterior axis specification
epithelial structure maintenance
Wnt signaling pathway
cerebellum development
cerebellar Purkinje cell differentiation
cellular component assembly
neuron differentiation
regulation of cell migration
positive regulation of transcription elongation from RNA polymerase II promoter
somatic stem cell population maintenance
regulation of kinase activity
negative regulation of erythrocyte differentiation
positive regulation of cell adhesion
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of hemoglobin biosynthetic process
regulation of focal adhesion assembly
head development
regulation of DNA-templated transcription, elongation
histone H3-K4 acetylation
regulation of nucleic acid-templated transcription
regulation of transcription from RNA polymerase II promoter
multicellular organism development
primitive erythrocyte differentiation
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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