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LDB1 Antibody (PA5-21577) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of LDB1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: LDB1 Polyclonal Antibody (Product # PA5-21577) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-21577
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 86 and 411 of LDB1 (Uniprot ID#Q86U70)
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11151936
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain.
Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (97%), Pig (98%), Chicken (97%), Rhesus Monkey (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
LIM domain-binding protein 1 (LDB1), also known as Carboxyl-terminal LIM domain-binding protein 2 (CLIM2), binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. LDB1 may regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. It plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. LDB1 acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. It also acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state. LDB1 strongly interacts with the LIM2 domain of LMX1A to form homodimer. LDB1 protein is expressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Due to alternative splicing events, three isoforms exist for LDB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: carboxy terminal LIM domain protein 2; Carboxyl-terminal LIM domain-binding protein 2; CLIM-2; hLdb1; LDB-1; LIM domain-binding factor CLIM2; LIM domain-binding factor-1; LIM domain-binding protein 1; mLdb1; Nuclear LIM interactor
Gene Aliases: CLIM-2; CLIM2; LDB-1; LDB1; NLI
UniProt ID: (Human) Q86U70, (Mouse) P70662
Entrez Gene ID: (Human) 8861, (Rat) 309447, (Mouse) 16825
RNA polymerase II activating transcription factor binding
enhancer sequence-specific DNA binding
chromatin binding
transcription corepressor activity
protein binding
enzyme binding
LIM domain binding
protein homodimerization activity
protein self-association
transcription factor activity, transcription factor binding
DNA binding
transcription cofactor activity
transcription cofactor
transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery
gastrulation with mouth forming second
hair follicle development
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
anterior/posterior axis specification
epithelial structure maintenance
Wnt signaling pathway
cerebellar Purkinje cell differentiation
cellular component assembly
neuron differentiation
regulation of cell migration
regulation of DNA-templated transcription, elongation
somatic stem cell population maintenance
regulation of kinase activity
histone H3-K4 acetylation
negative regulation of erythrocyte differentiation
positive regulation of cell adhesion
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of hemoglobin biosynthetic process
regulation of focal adhesion assembly
cerebellum development
head development
regulation of nucleic acid-templated transcription
regulation of transcription from RNA polymerase II promoter
primitive erythrocyte differentiation
Performance Guarantee
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