Proteintech

LDHA Polyclonal Antibody

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LDHA Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 and Rhodamine-Goat anti-Rabbit IGG.

LDHA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).

LDHA Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

LDHA Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug LDHA-Specific antibody (19987-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

LDHA Antibody in Immunoprecipitation (IP) — IP result of anti-LDHA-Specific (IP:19987-1-AP, 4ug; Detection:19987-1-AP 1:2000) with mouse kidney tissue lysate 4000ug.

Product Details

19987-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 29 publications 29 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Conjugate	Unconjugated
Form	Liquid
Concentration	0.26 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

The antibody has no cross reaction to LDHB and LDHC. This antibody is specific to LDHA.

Target Information

Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Bioinformatics

Protein Aliases: Cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; L-lactate dehydrogenase A chain; L-lactate dehydrogenase C chain; Lactate Dehydrogenase; lactate dehydrogenase 1, A chain; lactate dehydrogenase 3, C chain, sperm specific; lactate dehydrogenase A-like; lactate dehydrogenase A4; lactate dehydrogenase C variant 3; lactate dehydrogenase M; lactate dehydrogenase-A; Lactic Dehydrogenase; LDH muscle subunit; LDH testis subunit; LDH-A; LDH-C; LDH-M; LDH-X; M-LDH; OTTHUMP00000165206; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59

Gene Aliases: GSD11; HEL-S-133P; l7R2; Ldh-1; Ldh1; Ldh3; LDHA; LDHM; PIG19

UniProt ID: (Human) P00338, (Rat) P04642, (Mouse) P06151

Entrez Gene ID: (Human) 3939, (Rat) 24533, (Mouse) 16828, (Rat) 29634

Molecular Function: dehydrogenase