Proteintech

LDLR Polyclonal Antibody

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LDLR Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 10785-1-AP (LDLR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

LDLR Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain tissue slide using 10785-1-AP ( LDLR Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Sodium Citrate buffer (pH6).

LDLR Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

LDLR Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with .2ug LDLR antibody (10785-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

LDLR Antibody in Immunoprecipitation (IP) — IP result of anti-LDLR (IP:10785-1-AP, 5ug; Detection:10785-1-AP 1:500) with HeLa cells lysate 1200ug.

Product Details

10785-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 24 publications 24 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 1 publication 1 publication
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse
Published species	Chicken, Hamster, Human, Mouse, Pig, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LDLR Fusion Protein Ag1236 (1-350 aa encoded by BC014514) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.31 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MGPWGWKLR WTVALLLAAA GTAVGDRCER NEFQCQDGKC ISYKWVCDGS AECQDGSDES QETCLSVTCK SGDFSCGGRV NRCIPQFWRC DGQVDCDNGS DEQGCPPKTC SQDEFRCHDG KCISRQFVCD SDRDCLDGSD EASCPVLTCG PASFQCNSST CIPQLWACDN DPDCEDGSDE WPQRCRGLYV FQGDSSPCSA FEFHCLSGEC IHSSWRCDGG PDCKDKSDEE NCAVATCRPD EFQCSDGNCI HGSRQCDREY DCKDMSDEVG CVNVTLCEGP NKFKCHSGEC ITLDKVCNMA RDCRDWSDEP IKECGTNECL DNNGGCSHVC NDLKIGYECL CPDGFQLVAQ R (1-350 aa encoded by BC014514)

Target Information

The low density lipoprotein receptor (LDL-R) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in the LDL-R gene cause the autosomal dominant disorder, familial hypercholesterolemia. Along with SCARB1, CLDN1, and the tetraspanin superfamily member CD81, LDL-R has been reported to be an entry factor for the Hepatitis C virus. At least three isoforms of LDL-R are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cholesterol-lowering effects and potential mechanisms of chitooligosaccharide capsules in hyperlipidemic rats.

Food & nutrition research

Jiang Y,Fu C,Liu G,Guo J,Su Z

Fri Mar 18 00:00:00 EDT 2022

Endogenous cholesterol ester hydroperoxides modulate cholesterol levels and inhibit cholesterol uptake in hepatocytes and macrophages.

Redox biology

Guo S,Lu J,Zhuo Y,Xiao M,Xue X,Zhong S,Shen X,Yin C,Li L,Chen Q,Zhu M,Chen B,Zhao M,Zheng L,Tao Y,Yin H

Fri Feb 01 00:00:00 EST 2019

Endogenous cholesterol ester hydroperoxides modulate cholesterol levels and inhibit cholesterol uptake in hepatocytes and macrophages.

Redox biology

Guo S,Lu J,Zhuo Y,Xiao M,Xue X,Zhong S,Shen X,Yin C,Li L,Chen Q,Zhu M,Chen B,Zhao M,Zheng L,Tao Y,Yin H

Fri Feb 01 00:00:00 EST 2019

Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.

The Journal of clinical investigation

Zhong S,Li L,Zhang YL,Zhang L,Lu J,Guo S,Liang N,Ge J,Zhu M,Tao Y,Wu YC,Yin H

Wed Jan 02 00:00:00 EST 2019

Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.

The Journal of clinical investigation

Zhong S,Li L,Zhang YL,Zhang L,Lu J,Guo S,Liang N,Ge J,Zhu M,Tao Y,Wu YC,Yin H

Wed Jan 02 00:00:00 EST 2019

Berberrubine and its analog, hydroxypropyl-berberrubine, regulate LDLR and PCSK9 expression via the ERK signal pathway to exert cholesterol-lowering effects in human hepatoma HepG2 cells.

Journal of cellular biochemistry

Cao S,Xu P,Yan J,Liu H,Liu L,Cheng L,Qiu F,Kang N

Thu Oct 18 00:00:00 EDT 2018

Lead exposure induces Alzheimers's disease (AD)-like pathology and disturbes cholesterol metabolism in the young rat brain.

Toxicology letters

Zhou CC,Gao ZY,Wang J,Wu MQ,Hu S,Chen F,Liu JX,Pan H,Yan CH

Mon Oct 15 00:00:00 EDT 2018

Lead exposure induces Alzheimers's disease (AD)-like pathology and disturbes cholesterol metabolism in the young rat brain.

Toxicology letters

Zhou CC,Gao ZY,Wang J,Wu MQ,Hu S,Chen F,Liu JX,Pan H,Yan CH

Mon Oct 15 00:00:00 EDT 2018

Polydatin Improves Glucose and Lipid Metabolisms in Insulin-Resistant HepG2 Cells through the AMPK Pathway.

Biological & pharmaceutical bulletin

Hao J,Huang K,Chen C,Liang Y,Wang Y,Zhang X,Huang H

Thu Oct 04 00:00:00 EDT 2018

A Novel Role of Id1 in Regulating Oscillatory Shear Stress-Mediated Lipid Uptake in Endothelial Cells.

Annals of biomedical engineering

Zhang K,Chen Y,Zhang T,Huang L,Wang Y,Yin T,Qiu J,Gregersen H,Wang G

Fri Jun 01 00:00:00 EDT 2018

A Novel Role of Id1 in Regulating Oscillatory Shear Stress-Mediated Lipid Uptake in Endothelial Cells.

Annals of biomedical engineering

Zhang K,Chen Y,Zhang T,Huang L,Wang Y,Yin T,Qiu J,Gregersen H,Wang G

Fri Jun 01 00:00:00 EDT 2018

Dietary betaine supplementation in hens modulates hypothalamic expression of cholesterol metabolic genes in F1 cockerels through modification of DNA methylation.

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology

Idriss AA,Hu Y,Hou Z,Hu Y,Sun Q,Omer NA,Abobaker H,Ni Y,Zhao R

Thu Mar 01 00:00:00 EST 2018

Cavin-1 regulates caveolae-mediated LDL transcytosis: crosstalk in an AMPK/eNOS/ NF-κB/Sp1 loop.

Oncotarget

Bai XL,Yang XY,Li JY,Jia X,Xiong ZF,Wang YM,Jin S

Tue Nov 28 00:00:00 EST 2017

In Vivo Base Editing of PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) as a Therapeutic Alternative to Genome Editing.

Arteriosclerosis, thrombosis, and vascular biology

Chadwick AC,Wang X,Musunuru K

Fri Sep 01 00:00:00 EDT 2017

Reactive oxygen species mediate angiotensin II-induced transcytosis of low-density lipoprotein across endothelial cells.

International journal of molecular medicine

Bian F,Cui J,Zheng T,Jin S

Wed Mar 01 00:00:00 EST 2017

The antihypercholesterolemic effect of columbamine from Rhizoma Coptidis in HFHC-diet induced hamsters through HNF-4α/FTF-mediated CYP7A1 activation.

Fitoterapia

Wang Y,Han Y,Chai F,Xiang H,Huang T,Kou S,Han B,Gong X,Ye X

Thu Dec 01 00:00:00 EST 2016

Neglected but Important Role of Apolipoprotein E Exchange in Hepatitis C Virus Infection.

Journal of virology

Yang Z,Wang X,Chi X,Zhao F,Guo J,Ma P,Zhong J,Niu J,Pan X,Long G

Tue Nov 01 00:00:00 EDT 2016

Maternal Betaine Supplementation throughout Gestation and Lactation Modifies Hepatic Cholesterol Metabolic Genes in Weaning Piglets via AMPK/LXR-Mediated Pathway and Histone Modification.

Nutrients

Cai D,Yuan M,Liu H,Pan S,Ma W,Hong J,Zhao R

Tue Oct 18 00:00:00 EDT 2016

Downregulation of miR-150 Expression by DNA Hypermethylation Is Associated with High 2-Hydroxy-(4-methylthio)butanoic Acid-Induced Hepatic Cholesterol Accumulation in Nursery Piglets.

Journal of agricultural and food chemistry

Jia Y,Ling M,Zhang L,Jiang S,Sha Y,Zhao R

Wed Oct 12 00:00:00 EDT 2016

Rhizoma Coptidis alkaloids alleviate hyperlipidemia in B6 mice by modulating gut microbiota and bile acid pathways.

Biochimica et biophysica acta

He K,Hu Y,Ma H,Zou Z,Xiao Y,Yang Y,Feng M,Li X,Ye X

Thu Sep 01 00:00:00 EDT 2016

Hypolipidemic effects of Myrica rubra extracts and main compounds in C57BL/6j mice.

Food & function

He K,Li X,Xiao Y,Yong Y,Zhang Z,Li S,Zhou T,Yang D,Gao P,Xin X

Wed Aug 10 00:00:00 EDT 2016

Maternal sodium butyrate supplement elevates the lipolysis in adipose tissue and leads to lipid accumulation in offspring liver of weaning-age rats.

Lipids in health and disease

Zhou J,Gao S,Chen J,Zhao R,Yang X

Fri Jul 22 00:00:00 EDT 2016

Activation of epithelial proliferation induced by Eimeria acervulina infection in the duodenum may be associated with cholesterol metabolism.

Oncotarget

Sun L,Dong H,Zhang Z,Liu J,Hu Y,Ni Y,Grossmann R,Zhao R

Tue May 10 00:00:00 EDT 2016

Impact of age and sex on the development of atherosclerosis and expression of the related genes in apoE deficient mice.

Biochemical and biophysical research communications

Liu M,Zhang W,Li X,Han J,Chen Y,Duan Y

Fri Jan 15 00:00:00 EST 2016

Exogenous administration of chronic corticosterone affects hepatic cholesterol metabolism in broiler chickens showing long or short tonic immobility.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Liu J,Duan Y,Hu Y,Sun L,Wang S,Fu W,Ni Y,Zhao R

Fri Jan 01 00:00:00 EST 2016

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Scientific reports

Yuan ZY,Lu X,Lei F,Chai YS,Wang YG,Jiang JF,Feng TS,Wang XP,Yu X,Yan XJ,Xing DM,Du LJ

Wed Dec 16 00:00:00 EST 2015

The safety and anti-hypercholesterolemic effect of coptisine in Syrian golden hamsters.

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He K,Ye X,Wu H,Wang Y,Zou Z,Ning N,Hu Y,Chen B,Fang X,Li X

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Cholesterol deregulation induced by chronic corticosterone (CORT) stress in pectoralis major of broiler chickens.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Duan Y,Fu W,Wang S,Ni Y,Zhao R

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Journal of molecular and cellular cardiology

Zhang Y,Yang X,Bian F,Wu P,Xing S,Xu G,Li W,Chi J,Ouyang C,Zheng T,Wu D,Zhang Y,Li Y,Jin S

Tue Jul 01 00:00:00 EDT 2014

Danhong injection inhibits the development of atherosclerosis in both Apoe⁻/⁻ and Ldlr⁻/⁻ mice.

Journal of cardiovascular pharmacology

Chen Y,Liu M,Zhao T,Zhao B,Jia L,Zhu Y,Zhang B,Gao X,Li G,Li X,Xiang R,Han J,Duan Y

Thu May 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: LDL receptor; ldl-r; Low-density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3

Gene Aliases: FH; FHC; Hlb301; LDLCQ2; LDLR

UniProt ID: (Human) P01130, (Mouse) P35951

Entrez Gene ID: (Human) 3949, (Mouse) 16835

Molecular Function: apolipoprotein

Function(s)

lipid metabolic process endocytosis receptor-mediated endocytosis cholesterol metabolic process positive regulation of triglyceride biosynthetic process regulation of phosphatidylcholine catabolic process phospholipid transport intestinal cholesterol absorption cholesterol transport low-density lipoprotein particle clearance lipoprotein catabolic process cholesterol homeostasis viral entry into host cell cholesterol import regulation of cholesterol homeostasis transport lipid transport steroid metabolic process lipoprotein metabolic process

Process(es)

virus receptor activity glycoprotein binding protease binding receptor activity low-density lipoprotein receptor activity calcium ion binding protein binding low-density lipoprotein particle binding very-low-density lipoprotein particle receptor activity clathrin heavy chain binding identical protein binding

LDLR Polyclonal Antibody

Product Details

10785-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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