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Invitrogen

LEF1 Polyclonal Antibody

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LEF1 Polyclonal Antibody

Product Details

PA5-119910

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:100
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide within human LEF1 aa 150-200.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2913482

Product Specific Information

Positive Control: Jurkat cell lysates, human tonsil tissue, mouse colon tissue, mouse spleen tissue, HT-29.

Subcellular Location: Nucleus.

Target Information

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp586H0919; FLJ46390; HGNC:6551; hLEF-1 isoform; hLEF-1(8A); HMG Binding Domain; HMG box transcription factor; LEF-1; Lymphoid enhancer-binding factor 1; OTTHUMP00000219736; OTTHUMP00000219737; T cell-specific transcription factor 1-alpha; TCF1-alpha; unnamed protein product

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Gene Aliases: 3000002B05; AI451430; ECTD1; ECTD17; LEF-1; LEF1; TCF10; TCF1ALPHA; TCF7L3

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UniProt ID: (Human) Q3ZCU4, (Mouse) P27782

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Entrez Gene ID: (Human) 51176, (Mouse) 16842

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcription corepressor binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding beta-catenin binding DNA binding, bending estrogen receptor binding histone deacetylase binding sequence-specific DNA binding gamma-catenin binding armadillo repeat domain binding C2H2 zinc finger domain binding transcription regulator inhibitor activity sequence-specific double-stranded DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription factor binding estrogen receptor activity enhancer binding histone binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process transcription regulatory region DNA binding DNA-binding transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter patterning of blood vessels osteoblast differentiation somitogenesis epithelial to mesenchymal transition vasculature development sprouting angiogenesis regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter positive regulation of gene expression positive regulation of epithelial to mesenchymal transition Wnt signaling pathway dentate gyrus development hippocampus development forebrain radial glial cell differentiation forebrain neuroblast division formation of radial glial scaffolds regulation of cell-cell adhesion regulation of Wnt signaling pathway cell differentiation positive regulation of Wnt signaling pathway neutrophil differentiation embryonic limb morphogenesis positive regulation of cell migration BMP signaling pathway positive regulation of granulocyte differentiation mammary gland development negative regulation of interleukin-13 production negative regulation of interleukin-4 production negative regulation of interleukin-5 production T cell receptor V(D)J recombination B cell proliferation odontogenesis of dentin-containing tooth negative regulation of apoptotic process negative regulation of DNA binding tongue development positive regulation by host of viral transcription T-helper 1 cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of cell differentiation negative regulation of striated muscle tissue development negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter alpha-beta T cell differentiation paraxial mesoderm formation cell development regulation of neurogenesis sensory perception of taste anatomical structure regression canonical Wnt signaling pathway face morphogenesis cell chemotaxis apoptotic process involved in morphogenesis chorio-allantoic fusion trachea gland development secondary palate development protein localization to chromatin cellular response to cytokine stimulus cellular response to interleukin-4 positive regulation of cell proliferation in bone marrow negative regulation of apoptotic process in bone marrow positive regulation of cell cycle process positive regulation of odontoblast differentiation apoptotic process involved in patterning of blood vessels positive regulation of chondrocyte proliferation epithelial cell apoptotic process positive regulation of endothelial cell-matrix adhesion via fibronectin neural crest cell migration transcription, DNA-templated positive regulation of cell proliferation hypothalamus development forebrain neuron differentiation negative regulation of cell-cell adhesion positive regulation of cell-cell adhesion positive regulation of cell growth negative regulation of cysteine-type endopeptidase activity involved in apoptotic process steroid hormone mediated signaling pathway histone H3 acetylation histone H4 acetylation eye pigmentation generation of neurons muscle fiber development palate development odontoblast differentiation negative regulation of estrogen receptor binding negative regulation of canonical Wnt signaling pathway
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