Search
Invitrogen
IkB alpha Monoclonal Antibody (7A4)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 4
IkB alpha Antibody (LF-MA0235) in WB
Western Blot was performed using Anti-IkB alpha Monoclonal Antibody (7A4) (Product # LF-MA0235) and a 36 kDa band corresponding to NF-kappa-B inhibitor alpha was observed to be down regulated upon TNF alpha treatment in Hela, NIH/3T3 and LNCaP. Also, the protein expression was restored In LNCaP upon MG132 treatment. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with TNF alpha (20 ng/mL, 10 min) (Lane 2), NIH/3T3 (Lane 3), NIH/3T3 treated with TNF alpha (20 ng/mL, 10 min) (Lane 4), LNCaP (Lane 5), LNCaP treated wit... View More
Product Details
LF-MA0235
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
7A4
Immunogen
Recombinant human protein purified from E.coli(His/ABD-IkBα)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
Product Specific Information
A suggested positive control for this product is HeLa cells.
Target Information
IkB-alpha is a 40 kDa protein that functions to inhibit NF- kappaB activity. The inhibition occurs via protein-protein interaction between I kappaB proteins and NF- kappaB dimers in the cytosol. The interaction of I kappa B-alpha with NF- kappaB masks the nuclear localization sequence of NF- kappaB, preventing NF- kappaB translocation to the nucleus. A variety of stimuli can activate gene expression by liberating NF- kappaB through the degradation of I kappaB alpha. These stimuli include the proinflammatory cytokines TNF- alpha and IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, as well as various chemical and physical stresses. In humans, the gene is located on the q arm of chromosome 14. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase alpha (IKK alpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation Serines 32 and 36. IKK alpha appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma, and each appear to make essential contributions to IkB phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support