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LGP2 Polyclonal Antibody

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Antibody Testing Data (11)

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 11

LGP2 Antibody (11355-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11355-1-AP (LGP2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LGP2 Antibody in Western Blot (WB)

LGP2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11355-1-AP (LGP2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11355-1-AP (LGP2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11355-1-AP (LGP2 antibody) at dilution of 1:50 (under 40x lens).

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11355-1-AP (LGP2 antibody) at dilution of 1:50 (under 10x lens).

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11355-1-AP (LGP2 antibody) at dilution of 1:50 (under 40x lens).

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11355-1-AP (LGP2 antibody) at dilution of 1:50 (under 10x lens).

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11355-1-AP (LGP2 antibody) at dilution of 1:50 (under 40x lens).

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11355-1-AP (LGP2 antibody) at dilution of 1:50 (under 10x lens).

LGP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11355-1-AP (LGP2 antibody) at dilution of 1:10 (under 10x lens).

LGP2 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 11355-1-AP ( LGP2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

LGP2 Antibody in Immunoprecipitation (IP) — IP result of anti-LGP2 (IP:11355-1-AP, 3ug; Detection:11355-1-AP 1:300) with mouse liver tissue lysate 6000ug.

Product Details

11355-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Non-human primate, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

LGP2 Fusion Protein Ag1910 (330-678 aa encoded by BC014949)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LFDDRKNEL AHLATHGPEN PKLEMLEKIL QRQFSSSNSP RGIIFTRTRQ SAHSLLLWLQ QQQGLQTVDI RAQLLIGAGN SSQSTHMTQR DQQEVIQKFQ DGTLNLLVAT SVAEEGLDIP HCNVVVRYGL LTNEISMVQA RGRARADQSV YAFVATEGSR ELKRELINEA LETLMEQAVA AVQKMDQAEY QAKIRDLQQA ALTKRAAQAA QRENQRQQFP VEHVQLLCIN CMVAVGHGSD LRKVEGTHHV NVNPNFSNYY NVSRDPVVIN KVFKDWKPGG VISCRNCGEV WGLQMIYKSV KLPVLKVRSM LLETPQGRIQ AKKWSRVPFS VPDFDFLQHC AENLSDLSLD (330-678 aa encoded by BC014949)

Target Information

Anti-viral innate immune responses are triggered by pathogen-associated molecular patters (such as the accumulation of intracellular nucleic acids resulting from virus infections) and represent the first line of defense against numerous infectious organisms. The Toll-like receptors (TLR) 3, 7, 8, and 9 are expressed in immune cells and function as transmembrane pattern recognition receptors to detect foreign nucleic acids. Other proteins that play similar roles, such as RIG-1 and MDA5 are members of a CARD-helicase family and are expressed in the cytoplasm. A third protein, LGP2, is similar to RIG-1 and MDA5, except for lacking a homologous CARD domain. It is thought to act as an element of negative-feedback regulation of intracellular antiviral signaling. At least four isoforms of LGP2 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling.

Journal of virology

Zhu Z,Zhang M,Yuan L,Xu Y,Zhou H,Lian Z,Liu P,Li X

Tue Jan 31 00:00:00 EST 2023

LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling.

Journal of virology

Zhu Z,Zhang M,Yuan L,Xu Y,Zhou H,Lian Z,Liu P,Li X

Tue Jan 31 00:00:00 EST 2023

LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling.

Journal of virology

Zhu Z,Zhang M,Yuan L,Xu Y,Zhou H,Lian Z,Liu P,Li X

Tue Jan 31 00:00:00 EST 2023

LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling.

Journal of virology

Zhu Z,Zhang M,Yuan L,Xu Y,Zhou H,Lian Z,Liu P,Li X

Tue Jan 31 00:00:00 EST 2023

LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling.

Journal of virology

Zhu Z,Zhang M,Yuan L,Xu Y,Zhou H,Lian Z,Liu P,Li X

Tue Jan 31 00:00:00 EST 2023

LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling.

Journal of virology

Zhu Z,Zhang M,Yuan L,Xu Y,Zhou H,Lian Z,Liu P,Li X

Tue Jan 31 00:00:00 EST 2023

LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling.

Journal of virology

Zhu Z,Zhang M,Yuan L,Xu Y,Zhou H,Lian Z,Liu P,Li X

Tue Jan 31 00:00:00 EST 2023

LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling.

Journal of virology

Zhu Z,Zhang M,Yuan L,Xu Y,Zhou H,Lian Z,Liu P,Li X

Tue Jan 31 00:00:00 EST 2023

LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling.

Journal of virology

Zhu Z,Zhang M,Yuan L,Xu Y,Zhou H,Lian Z,Liu P,Li X

Tue Jan 31 00:00:00 EST 2023

PKR Binds Enterovirus IRESs, Displaces Host Translation Factors, and Impairs Viral Translation to Enable Innate Antiviral Signaling.

mBio

Dobrikov MI,Dobrikova EY,McKay ZP,Kastan JP,Brown MC,Gromeier M

Tue Jun 28 00:00:00 EDT 2022

Immune regulator LGP2 targets Ubc13/UBE2N to mediate widespread interference with K63 polyubiquitination and NF-κB activation.

Cell reports

Lenoir JJ,Parisien JP,Horvath CM

Tue Dec 28 00:00:00 EST 2021

DExH-Box helicase 58 enhances osteoblast differentiation of osteoblastic cells via Wnt/β-Catenin signaling.

Biochemical and biophysical research communications

Zhou L,Sun S,Xu L,Yu Y,Zhang T,Wang M

Tue Apr 02 00:00:00 EDT 2019

RIG-I-like receptors direct inflammatory macrophage polarization against West Nile virus infection.

Nature communications

Stone AEL,Green R,Wilkins C,Hemann EA,Gale M

Tue Aug 13 00:00:00 EDT 2019

miR-122 does not impact recognition of the HCV genome by innate sensors of RNA but rather protects the 5' end from the cellular pyrophosphatases, DOM3Z and DUSP11.

Nucleic acids research

Amador-Cañizares Y,Bernier A,Wilson JA,Sagan SM

Fri Jun 01 00:00:00 EDT 2018

miR-122 does not impact recognition of the HCV genome by innate sensors of RNA but rather protects the 5' end from the cellular pyrophosphatases, DOM3Z and DUSP11.

Nucleic acids research

Amador-Cañizares Y,Bernier A,Wilson JA,Sagan SM

Fri Jun 01 00:00:00 EDT 2018

RNA sensor LGP2 inhibits TRAF ubiquitin ligase to negatively regulate innate immune signaling.

EMBO reports

Parisien JP,Lenoir JJ,Mandhana R,Rodriguez KR,Qian K,Bruns AM,Horvath CM

Fri Jun 01 00:00:00 EDT 2018

RNA sensor LGP2 inhibits TRAF ubiquitin ligase to negatively regulate innate immune signaling.

EMBO reports

Parisien JP,Lenoir JJ,Mandhana R,Rodriguez KR,Qian K,Bruns AM,Horvath CM

Fri Jun 01 00:00:00 EDT 2018

Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma.

Cancer science

Lin LL,Huang CC,Wu MT,Hsu WM,Chuang JH

Thu Nov 01 00:00:00 EDT 2018

Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma.

Cancer science

Lin LL,Huang CC,Wu MT,Hsu WM,Chuang JH

Thu Nov 01 00:00:00 EDT 2018

Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma.

Cancer science

Lin LL,Huang CC,Wu MT,Hsu WM,Chuang JH

Thu Nov 01 00:00:00 EDT 2018

Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma.

Cancer science

Lin LL,Huang CC,Wu MT,Hsu WM,Chuang JH

Thu Nov 01 00:00:00 EDT 2018

Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma.

Cancer science

Lin LL,Huang CC,Wu MT,Hsu WM,Chuang JH

Thu Nov 01 00:00:00 EDT 2018

Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma.

Cancer science

Lin LL,Huang CC,Wu MT,Hsu WM,Chuang JH

Thu Nov 01 00:00:00 EDT 2018

The TAR-RNA binding protein is required for immunoresponses triggered by Cardiovirus infection.

Biochemical and biophysical research communications

Komuro A,Homma Y,Negoro T,Barber GN,Horvath CM

Fri Nov 11 00:00:00 EST 2016

The TAR-RNA binding protein is required for immunoresponses triggered by Cardiovirus infection.

Biochemical and biophysical research communications

Komuro A,Homma Y,Negoro T,Barber GN,Horvath CM

Fri Nov 11 00:00:00 EST 2016

Ambivalent role of the innate immune response in rabies virus pathogenesis.

Journal of virology

Chopy D,Pothlichet J,Lafage M,Mégret F,Fiette L,Si-Tahar M,Lafon M

Fri Jul 01 00:00:00 EDT 2011

Ambivalent role of the innate immune response in rabies virus pathogenesis.

Journal of virology

Chopy D,Pothlichet J,Lafage M,Mégret F,Fiette L,Si-Tahar M,Lafon M

Fri Jul 01 00:00:00 EDT 2011

Ambivalent role of the innate immune response in rabies virus pathogenesis.

Journal of virology

Chopy D,Pothlichet J,Lafage M,Mégret F,Fiette L,Si-Tahar M,Lafon M

Fri Jul 01 00:00:00 EDT 2011

The RIG-I-like receptor LGP2 recognizes the termini of double-stranded RNA.

The Journal of biological chemistry

Li X,Ranjith-Kumar CT,Brooks MT,Dharmaiah S,Herr AB,Kao C,Li P

Fri May 15 00:00:00 EDT 2009

Characterization of retinoic acid-inducible gene-I expression in primary murine glia following exposure to vesicular stomatitis virus.

Journal of neurovirology

Furr SR,Chauhan VS,Sterka D,Grdzelishvili V,Marriott I

Sat Nov 01 00:00:00 EDT 2008

Characterization of retinoic acid-inducible gene-I expression in primary murine glia following exposure to vesicular stomatitis virus.

Journal of neurovirology

Furr SR,Chauhan VS,Sterka D,Grdzelishvili V,Marriott I

Sat Nov 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: ATP-dependent helicase LGP2; ATP-dependent RNA helicase DHX58; DEXH (Asp-Glu-X-His) box polypeptide 58; ortholog of mouse D11lgp2; probable ATP-dependent helicase LGP2; probable ATP-dependent RNA helicase DHX58; Protein D11Lgp2; Protein D11Lgp2 homolog; RIG-I-like receptor 3; RIG-I-like receptor LGP2; RLR; RLR-3; RNA helicase LGP2

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Gene Aliases: B430001I08Rik; D11LGP2; D11LGP2E; DHX58; LGP2; LPG2; RGD1310093; RLR-3

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UniProt ID: (Human) Q96C10, (Mouse) Q99J87

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Entrez Gene ID: (Human) 79132, (Mouse) 80861, (Rat) 303538

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DNA binding double-stranded RNA binding single-stranded RNA binding helicase activity protein binding ATP binding zinc ion binding nucleotide binding RNA binding hydrolase activity hydrolase activity, acting on acid anhydrides metal ion binding

Process(es)

response to virus negative regulation of type I interferon production positive regulation of type I interferon production negative regulation of MDA-5 signaling pathway negative regulation of RIG-I signaling pathway innate immune response regulation of innate immune response negative regulation of innate immune response defense response to virus positive regulation of MDA-5 signaling pathway positive regulation of RIG-I signaling pathway immune system process metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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