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Agrisera
LHCSR3 Polyclonal Antibody
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FIGURE: 1 / 1
LHCSR3 Antibody (AS14-2766) in WB
Following samples: 0.1 µg of Lhc SR 3 IB + His Tag (1), 0.05 µg of Lhc SR 3 IB + His Tag (2), 5 µg of Chlamydomonas reinhardtii wild type (CC 124) total protein extract of photo autotrophically grown cells in light intensity: 60 µE (3), 5 µg of Chlamydomonas reinhardtii wild type (CC 124) total protein extract of photo autotrophically grown cells in high light intensity: 500 µE (4) were separated on 15% Tris-Glycine SDS PAGE and blotted overnight to PVDF using tank transfer. Blots were blocked with 5% BSA/milk for 1 h at room temperature (... View More
Product Details
AS14-2766
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Algae
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from LHCSR3 protein sequence from Chlamydomonas reinhardtii.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Bryopsis corticulans, Chlamydomonas reinhardtii, Nannochloropsis gaditana
Target Information
LHCSR3 encodes a stress-induced light-harvesting complex family protein that accumulates in the chloroplast thylakoid membrane of the green alga Chlamydomonas reinhardtii, where it associates with photosynthetic antenna supercomplexes under high-light and other photooxidative stress conditions. The mature polypeptide is an integral membrane, chlorophyll a/b- and carotenoid-binding antenna-like protein that adopts the canonical LHC fold with three transmembrane alpha-helices and additional lumen-exposed amphipathic helices and C-terminal regions enriched in protonatable acidic residues; specific lumen-facing Asp and Glu residues are required for sensing thylakoid lumen acidification. Functionally, LHCSR3 is essential for rapid, energy-dependent nonphotochemical quenching (qE), acting both as a pH-responsive trigger and as a direct excitation-energy quencher that converts excess absorbed light energy into heat, thereby limiting formation of reactive oxygen species and protecting photosystems from photoinhibition; mechanistic studies support distinct quenching components controlled by low pH and by xanthophyll composition, consistent with LHCSR3 providing flexible photoprotection across changing light and metabolic conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: LhcSR3; LHCSR3.1; LHCSR3.2; LI818
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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