Invitrogen

LHX2 Polyclonal Antibody

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LHX2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemichal analysis of LHX2 in Hela cells using a Polyclonal antibody (Product # PA5-112597) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.

LHX2 Antibody in Western Blot (WB) — Western Blot analysis of LHX2 in rat kidney tissue using a LHX2 polyclonal antibody (Product # PA5-112597) at a concentration of 5.43 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000.

Product Details

PA5-112597

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human LIM/homeobox protein Lhx2 protein (197-377AA)

Conjugate

Unconjugated

Form

Liquid

Concentration

2.72 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% ProClin 300

Storage conditions

-20°C or -80°C if preferred

RRID

AB_2867332

Target Information

This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Homeobox protein LH-2; LIM homeobox protein 2; LIM HOX gene 2; LIM/homeobox protein Lhx2; MGC138390

Gene Aliases: hLhx2; Lh-2; LH2; LH2A; LHX2

UniProt ID: (Human) P50458

Entrez Gene ID: (Human) 9355, (Rat) 296706

Molecular Function: helix-turn-helix transcription factor homeodomain transcription factor DNA-binding transcription factor gene-specific transcriptional regulator

neural tube closure hair follicle development transcription, DNA-templated axon guidance mesoderm development dorsal/ventral pattern formation olfactory bulb development telencephalon regionalization cerebral cortex development maintenance of epithelial cell apical/basal polarity positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter axon extension retina development in camera-type eye negative regulation of transcription regulatory region DNA binding regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter nervous system development brain development telencephalon development neurogenesis neuron differentiation anatomical structure formation involved in morphogenesis

Process(es)

transcription factor activity, RNA polymerase II transcription factor binding chromatin binding zinc ion binding sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding