Invitrogen

LHX2 Polyclonal Antibody

View all (30) LHX2 antibodies

Questions & Answers

Questions & Answers

Product Image Gallery

LHX2 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry (Frozen) analysis of LHX2 was performed in frozen-sectioned rat E13.5 brain tissue using LHX2 Polyclonal Antibody (Product # PA5-78287) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

LHX2 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry analysis of LHX2 in frozen adult mouse retina. Sample was incubated with LHX2 polyclonal antibody (Product # PA5-78287) using a dilution of 1:250 followed by beta tubulin 3 (red) and DAPI (blue) at a dilution of 1:250.

LHX2 Antibody in Western Blot (WB) — Western blot analysis of LHX2 in A) Neuro2A whole cell lysate, B) GL261 whole cell lysate, C) C8D30 whole cell lysate, D) NIH-3T3 whole cell lysate, E) BCL-1 whole cell lysate, F) BCL-1 whole cell lysate, G) Raw264.7 whole cell lysate, H) C2C12 whole cell lysate. Samples were separated with 10% SDS-PAGE and incubated with LHX2 polyclonal antibody (Product # PA5-78287) using a dilution of 1:1000.

LHX2 Antibody in Western Blot (WB) — Western blot analysis of LHX2 in various tissue extracts using 50 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with LHX2 polyclonal antibody (Product # PA5-78287) using a dilution of 1:10,000 (left panel) and 1:5000 (right panel) followed by HRP-conjugated anti-rabbit IgG.

LHX2 Antibody in Western Blot (WB) — Western blot analysis of LHX2 in A) 293T whole cell lysate, B) A431 whole cell lysate, C) HeLa D HepG2 whole cell lysate. Samples were separated with 10% SDS-PAGE and incubated with LHX2 polyclonal antibody (Product # PA5-78287) using a dilution of 1:1000.

Product Details

PA5-78287

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:20,000	-
Immunohistochemistry (Frozen) (IHC (F))	1:100-1:1,000	-

Product Specifications
Species Reactivity	Rat, Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein encompassing a sequence within the C-terminus region of human LHX2. The exact sequence is proprietary. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.97 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2736088

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, mouse brain, mouse eye

Predicted Reactivity: Pig (98%), Chicken (81%), Rhesus Monkey (98%), Bovine (98%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: Homeobox protein LH-2; LIM homeo box protein 2; LIM homeobox protein 2; LIM HOX gene 2; LIM/homeobox protein Lhx2; MGC138390

Gene Aliases: ap; apterous; hLhx2; Lh-2; LH2; LH2A; LHX2; Lim2

UniProt ID: (Human) P50458, (Mouse) Q9Z0S2

Entrez Gene ID: (Human) 9355, (Mouse) 16870, (Rat) 296706

Molecular Function: helix-turn-helix transcription factor homeodomain transcription factor DNA-binding transcription factor gene-specific transcriptional regulator

neural tube closure hair follicle development transcription, DNA-templated axon guidance mesoderm development dorsal/ventral pattern formation olfactory bulb development telencephalon regionalization cerebral cortex development maintenance of epithelial cell apical/basal polarity positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter axon extension retina development in camera-type eye negative regulation of transcription regulatory region DNA binding regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter nervous system development brain development telencephalon development neurogenesis neuron differentiation anatomical structure formation involved in morphogenesis

Process(es)

transcription factor activity, RNA polymerase II transcription factor binding chromatin binding zinc ion binding sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding DNA binding protein binding metal ion binding