Invitrogen

LHX2 Polyclonal Antibody

Product Image Gallery

LHX2 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry analysis of LHX2 in frozen adult mouse retina. Sample was incubated with LHX2 polyclonal antibody (Product # PA5-78287) using a dilution of 1:250 followed by beta tubulin 3 (red) and DAPI (blue) at a dilution of 1:250.

LHX2 Antibody in Western Blot (WB) — Western blot analysis of LHX2 in A) 293T whole cell lysate, B) A431 whole cell lysate, C) HeLa D HepG2 whole cell lysate. Samples were separated with 10% SDS-PAGE and incubated with LHX2 polyclonal antibody (Product # PA5-78287) using a dilution of 1:1000.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1000

Western Blot (WB)

1:5000-1:10,000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human LHX2. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2736088

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, mouse brain, mouse eye

Predicted Reactivity: Pig (98%), Chicken (81%), Rhesus Monkey (98%), Bovine (98%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

LHX2 encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: hLhx2; Homeobox protein LH-2; LIM homeo box protein 2; LIM homeobox 2; LIM homeobox protein 2; LIM HOX gene 2; LIM/homeobox protein Lhx2

Gene Aliases: ap; apterous; hLhx2; Lh-2; LH2; LH2A; LHX2; Lim2

UniProt ID: (Human) P50458, (Mouse) Q9Z0S2

Entrez Gene ID: (Human) 9355, (Mouse) 16870

Molecular Function: actin family cytoskeletal protein cytoskeletal protein