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Invitrogen
LIF Polyclonal Antibody
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FIGURE: 1 / 7
LIF Antibody (PA5-79600) in ICC/IF
Immunofluorescence analysis of LIF was performed using 80% confluent log phase MDA-MB-231 and T-47D, control and PTI treated, cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with LIF Polyclonal Antibody (Product # PA5-79600) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa F... View More
Product Details
PA5-79600
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human LIF recombinant protein (Position: S23-F202).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human placenta tissue, human A431 whole cell, human A549 whole cell. IHC: human lung cancer tissue, human placenta tissue.
Target Information
Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cholinergic differentiation factor; D factor; differentiation inhibitory activity; differentiation-inducing factor; Differentiation-stimulating factor; hepatocyte-stimulating factor III; human interleukin in DA cells; Leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI; unnamed protein product
Gene Aliases: CDF; DIA; HILDA; LIF; MLPLI
UniProt ID: (Human) P15018
Entrez Gene ID: (Human) 3976
cell morphogenesis
response to hypoxia
blood vessel remodeling
immune response
embryo implantation
cell proliferation
positive regulation of cell proliferation
negative regulation of cell proliferation
gene expression
positive regulation of gene expression
regulation of cell communication
regulation of signaling
cell differentiation
macrophage differentiation
lung development
positive regulation of peptidyl-serine phosphorylation
positive regulation of cell adhesion mediated by integrin
somatic stem cell population maintenance
positive regulation of MAPK cascade
regulation of cell differentiation
positive regulation of macrophage differentiation
negative regulation of meiotic nuclear division
positive regulation of transcription from RNA polymerase II promoter
decidualization
negative regulation of hormone secretion
fibroblast proliferation
positive regulation of fibroblast proliferation
lung alveolus development
muscle organ morphogenesis
neuron development
positive regulation of astrocyte differentiation
leukemia inhibitory factor signaling pathway
stem cell differentiation
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of multicellular organismal process
maternal process involved in female pregnancy
lung vasculature development
lung lobe morphogenesis
trophoblast giant cell differentiation
negative regulation of ERK1 and ERK2 cascade
positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
regulation of metanephric nephron tubule epithelial cell differentiation
receptor signaling pathway via STAT
meiotic nuclear division
positive regulation of STAT cascade
regulation of multicellular organismal development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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