|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptides corresponding to residues 149-165, 175-162, and 395-412 of human Lim-1.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: human muscle tissue, human skeletal muscle protein.
The LIM motif defines a zinc-binding domain that is found in a variety of transcriptional regulators and proto-oncogenes products. LIM domain mediates protein-protein interactions and, with LIM-homeodomain proteins, act as negative regulators of the transcriptional activation function of the protein. This transcription factor is required for embryonic survival and for the determination of many cell types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.