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Bethyl Laboratories
LIMCH1 Polyclonal Antibody
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FIGURE: 1 / 1
LIMCH1 Antibody (A304-933A) in WB
Detection of human LIMCH1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LIMCH1 antibody (Product # A304-933A) (lot A304-933A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A304-933A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 250 to 300 of human LIM and calponin homology domains-containing protein 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: LIM and calponin homology domains-containing protein 1; unnamed protein product
Gene Aliases: KIAA1102; LIMCH1; LIMCH1A; LMO7B
UniProt ID: (Human) Q9UPQ0
Entrez Gene ID: (Human) 22998
positive regulation of protein phosphorylation
positive regulation of macromolecule metabolic process
negative regulation of cell migration
actomyosin structure organization
positive regulation of stress fiber assembly
regulation of focal adhesion assembly
cytoplasmic actin-based contraction involved in cell motility
regulation of primary metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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