Immunofluorescent analysis of LIMD1 showing staining in the cytoplasm and nucleus of A431 cells. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a LIMD1 polyclonal antibody (Product # PA5-28491) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 472 and 676 of Human LIMD1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28491 targets LIMD1 in IHC (P) and WB applications and shows reactivity with Human and mouse samples.
The PA5-28491 immunogen is recombinant fragment corresponding to a region within amino acids 472 and 676 of Human LIMD1.
Transcriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.