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Invitrogen
LIME Polyclonal Antibody
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FIGURE: 1 / 1
LIME Antibody (PA5-89384) in WB
Western blot analysis of LIME in extracts of various cell lines. Samples were incubated with LIME Polyclonal antibody (Product # PA5-89384) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-89384
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human LIME1 (NP_0602762)
Conjugate
Form
Liquid
Concentration
1.08 mg/mL
Amount
108 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MGLPVSWAPP ALWVLGCCAL LLSLWALCTA CRRPEDAVAP RKRARRQRAR LQGSATAAEA SLLRRTHLCS LSKSDTRLHE LHRGPRSSRA LRPASMDLLR PHWLEVSRDI TGPQAAPSAF PHQELPRALP AAAATAGCAG LEATYSNVGL AALPGVSLAA SPVVAEYARV QKRKGTHRSP QEPQQGKTEV TPAAQVDVLY SRVCKPKRRD PGPTTDPLDP KGQGAILALA GDLAYQTLPL RALDVDSGPL ENVYESIREL GDPAGRSSTC GAGTPPASSC PSLGRGWRPL PASLP; Positive Samples: K-562, Mouse lung; Cellular Location: Cell membrane, Single-pass type III membrane protein
Target Information
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ20406; Lck-interacting membrane protein; Lck-interacting molecule; Lck-interacting transmembrane adapter 1; lime 1; RP4-583P15.5; unnamed protein product
Gene Aliases: 2810038K19Rik; dJ583P15.4; LIME; LIME1; LP8067
UniProt ID: (Human) Q9H400, (Mouse) Q9EQR5
Entrez Gene ID: (Human) 54923, (Mouse) 72699
adaptive immune response
immune system process
regulation of transcription from RNA polymerase II promoter
regulation of I-kappaB kinase/NF-kappaB signaling
T cell receptor signaling pathway
B cell receptor signaling pathway
regulation of release of sequestered calcium ion into cytosol
regulation of protein kinase B signaling
regulation of NIK/NF-kappaB signaling
regulation of phosphatidylinositol 3-kinase signaling
regulation of NF-kappaB import into nucleus
regulation of MAP kinase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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