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Invitrogen
LIMK1 Polyclonal Antibody
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FIGURE: 1 / 6
LIMK1 Antibody (PA5-14938) in ICC/IF
Immunofluorescence analysis of LIMK1 was performed using 70% confluent log phase PC-12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with LIMK1 Polyclonal Antibody (Product # PA5-14938) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A270... View More
Product Details
PA5-14938
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LIM Kinase 1 (LIMK1)
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
Target Information
LIMK1 is a serine/threonine kinase involved in regulation of actin cytoskeletal changes via phosphorylation of cofilin. LIMK1 has also been implicated in brain development. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EC 2.7.11.1; kinase LIMK1; LIM domain kinase 1; lim kinase 1; LIM motif-containing protein kinase; LIMK-1
Gene Aliases: LIMK; LIMK-1; LIMK1
UniProt ID: (Human) P53667
Entrez Gene ID: (Human) 3984
protein phosphorylation
signal transduction
Rho protein signal transduction
nervous system development
actin cytoskeleton organization
positive regulation of actin filament bundle assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
positive regulation of axon extension
negative regulation of ubiquitin-protein transferase activity
positive regulation of stress fiber assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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