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Immunofluorescence analysis of VELI-3 was performed using 70% confluent log phase RSC96 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with LIN7C Rabbit Polyclonal Antibody (515600) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Dog, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminus of the rat Velis-3 (MALS-3) protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1.0 ug/ml|
|Immunoprecipitation (IP)||1 ug|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Protein assembly at the post synaptic density (PSD) of neuronal synapses is mediated in part by protein interactions with PDZ (PSD-95/discs large/zona occludens-1) motifs.The MAmamalian LIN-7 (MALS) proteins are a family of small synaptic proteins that are the mammalian homologs of LIN-7, a C. elegans protein essential for vulval development. MALS proteins are also known as Veli (Vertebrate Lin-7) proteins. MALS-3 mRNA is most abundant in kidney, followed by brain, liver, thymus and heart. MALS-3 is undetectable in spleen. In cultured cortical neurons, MALS proteins are clustered together with PSD-95 and NMDA-type glutamate receptors. MALS proteins are suggested to have roles in regulating the recruitment of neurotransmitter receptors to the PSD and in the assembly of proteins involved in synaptic vesicle exocytosis and synaptic junctions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
51-5600 was used in immunohistochemistry - frozen section to identify specializations in murine ribbon synaptic endocytosis during different states of activity.
|Fuchs M,Brandstätter JH,Regus-Leidig H||Frontiers in cellular neuroscience (8:null)||2014|
miR-199a-5p regulates urothelial permeability and may play a role in bladder pain syndrome.
51-5600 was used in western blot to show that miR-99a-5p regulates intercellular junctions in the bladder.
|Monastyrskaya K,Sánchez-Freire V,Hashemi Gheinani A,Klumpp DJ,Babiychuk EB,Draeger A,Burkhard FC||The American journal of pathology (182:431)||2013|
|Mouse||Not Cited||Type 4 OFF cone bipolar cells of the mouse retina express calsenilin and contact cones as well as rods.||Haverkamp S,Specht D,Majumdar S,Zaidi NF,Brandstätter JH,Wasco W,Wässle H,Tom Dieck S||The Journal of comparative neurology (507:1087)||2008|
lin 7 homolog C; lin-7 homolog C; LIN-7 protein 3; lin-7-C; Lin-7C; LIN7C; MALS-3; MALS3; Mammalian lin-seven protein 3; mLin7C; Protein lin-7 homolog C; Protein lin-7-like protein C; Veli-3; veli-3 protein; VELI3; vertebrate homolog of C. elegans Lin-7 type 3; Vertebrate lin-7 homolog 3
9130007B12Rik; AI303698; AU019331; AW125731; D2Ertd520e; EGK_06400; LIN-7-C; LIN-7C; LIN7C; MALS-3; MALS3; VELI3