Invitrogen

LIS1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

1 Reference

View all (10) LIS1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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LIS1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry of LIS1 in Jurkat cells with LIS1 Polyclonal Antibody (Product # PA5-20419) at 2.5 µg/mL.

LIS1 Antibody in Western Blot (WB) — Western Blot analysis of LIS1 in HeLa cell lysate with LIS1 Polyclonal Antibody (Product # PA5-20419) at (A) 0.5 and (B) 1 µg/mL.

LIS1 Antibody — Effects of siRNA-mediated knockdown of LIS1 on CCA cell proliferation, invasion, and migration. A. Western blot was used to detect the expression of LIS1, AKT, p-AKT, and MMP2 in HCCC-9810 and CCLP1 cells transfected with indicated siRNAs (20 nmol/L) 48 h after transfection. B and C. The cell proliferation curve of HCCC-9810 and CCLP1 cells transfected with LIS1 siRNA or control for 48 h. D. Representative results of invasion assays. The bar graph showed quantification of cell invasion between cells transfected with LIS1 siRNA and control. E. The mRNA levels of LIS1 were detected in CCA tumor tissues or matched adjacent tissue by q-PCR.GAPDH was used as a internal control. F. Expression of miR-144 was inversely correlated with LIS1 in CCA tissues. Statistical analysis was performed using Pearson's correlation coefficient. (R2 = 0.49, P <0.01). *P < 0.05, **P <0.01. {KD}

Product Details

PA5-20419

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5-1 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	2.5 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A 14 amino acid peptide from near the carboxy terminus of human LIS1. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
RRID	AB_11154172

Product Specific Information

A suggested positive control is Hela cell lysate.

PA5-20419 can be used with blocking peptide PEP-0536.

Target Information

VAMP7 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family, localizing to late endosomes and lysosomes. VAMP7 is thought to mediate the fusion of endosomes to their target lysosomes as well as other exocytosis events during phagocytosis and neuritogenesis. VAMP7 interacts with the VPS9 ankyrin repeat protein VARP, a protein that localizes to early endosomes and thought to regulate endosome dynamics. Together with CD82, VAMP7 can modulate the signaling of EGFR by regulating its endocytosis from the plasma membrane.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MicroRNA-144 suppresses cholangiocarcinoma cell proliferation and invasion through targeting platelet activating factor acetylhydrolase isoform 1b.

BMC cancer

Yang R,Chen Y,Tang C,Li H,Wang B,Yan Q,Hu J,Zou S

PA5-20419 was used in western blot to investigate the role of microRNAs in cholangiocarcinoma

Fri Dec 05 00:00:00 EST 2014

Bioinformatics

Protein Aliases: LIS-1; LIS-1 {ECO:0000255|HAMAP-Rule:MF_03141}; lissencephaly 1 protein; Lissencephaly-1 protein; lissencephaly-1 protein {ECO:0000255|HAMAP-Rule:MF_03141}; MGC25297; PAF acetylhydrolase 45 kDa subunit; PAF acetylhydrolase 45 kDa subunit {ECO:0000255|HAMAP-Rule:MF_03141}; PAF-AH 45 kDa subunit; PAF-AH 45 kDa subunit {ECO:0000255|HAMAP-Rule:MF_03141}; PAF-AH alpha; PAF-AH alpha {ECO:0000255|HAMAP-Rule:MF_03141}; PAF-AH beta; PAFAH alpha; PAFAH alpha {ECO:0000255|HAMAP-Rule:MF_03141}; PAFAH1B1; platelet-activating factor acetylhydrolase 1b, regulatory subunit 1; platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa); platelet-activating factor acetylhydrolase beta subunit (PAF-AH beta); Platelet-activating factor acetylhydrolase IB subunit alpha; platelet-activating factor acetylhydrolase IB subunit alpha {ECO:0000255|HAMAP-Rule:MF_03141}; Platelet-activating factor acetylhydrolase IB subunit beta; platelet-activating factor acetylhydrolase, isoform 1b, beta1 subunit; platelet-activating factor acetylhydrolase, isoform 1b, subunit 1; platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD); platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa; platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa); RP23-194P5.2

Gene Aliases: LIS-1; LIS1; LIS2; MDCR; MDS; Mdsh; MMS10-U; Ms10u; NudF; PAFAH; PAFAH1B1; PAFAHA

UniProt ID: (Human) P43034, (Rat) P63004, (Mouse) P63005

Entrez Gene ID: (Human) 5048, (Rat) 83572, (Mouse) 18472

Function(s)

establishment of mitotic spindle orientation microtubule cytoskeleton organization ameboidal-type cell migration acrosome assembly neuron migration positive regulation of cytokine-mediated signaling pathway microtubule-based process mitotic nuclear division nuclear migration chemical synaptic transmission neuroblast proliferation brain development learning or memory retrograde axonal transport adult locomotory behavior protein secretion negative regulation of neuron projection development lipid catabolic process cell migration stem cell division transmission of nerve impulse corpus callosum morphogenesis hippocampus development layer formation in cerebral cortex cerebral cortex neuron differentiation cerebral cortex development actin cytoskeleton organization microtubule organizing center organization osteoclast development positive regulation of axon extension positive regulation of mitotic cell cycle negative regulation of JNK cascade vesicle transport along microtubule brain morphogenesis neuromuscular process controlling balance nuclear envelope disassembly establishment of centrosome localization lipid metabolic process transport cell cycle multicellular organism development nervous system development cell differentiation regulation of GTPase activity cell division G2/M transition of mitotic cell cycle sister chromatid cohesion germ cell development positive regulation of embryonic development establishment of planar polarity of embryonic epithelium cortical microtubule organization platelet activating factor metabolic process auditory receptor cell development positive regulation of dendritic spine morphogenesis regulation of microtubule cytoskeleton organization cochlea development microtubule cytoskeleton organization involved in establishment of planar polarity regulation of microtubule motor activity

Process(es)

phospholipase A2 activity protein binding microtubule binding heparin binding protein complex binding dynactin binding protein homodimerization activity phospholipase binding dynein binding dynein intermediate chain binding phosphoprotein binding platelet-activating factor acetyltransferase activity

LIS1 Polyclonal Antibody

Product Details

PA5-20419

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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