Invitrogen

LLGL1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

3 Published Figures

1 Reference

View all (6) LLGL1 antibodies

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Datasheet

Protocols

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LLGL1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of LLGL1 in human placenta using a LLGL1 Polyclonal Antibody (Product # PA5-54637) shows moderate cytoplasmic positivity in trophoblastic cells.

LLGL1 Antibody in Western Blot (WB) — Western blot analysis of LLGL1 in human cell line U-251 MG using a LLGL1 Polyclonal Antibody (Product # PA5-54637).

LLGL1 Antibody in Immunocytochemistry (ICC/IF) — Figure 10. Polarity markers in DLD20-2 cells expressing different sLUR mutants. (a) Representative z-sections of DLD20-2 cells expressing sLUR-517GFP, sLUR-469GFP, sLUR-402GFP, and sLUR-Delta(403-420). The cells were imaged for GFP fluorescence (green) in conjunction with immunostaining for Dlg1, Par6B, and Pals1 (all red). Bars, 10 um. Note that neither of the sLUR-517GFP mutants rescues ABP of DLD20-2 cells. (b) The cells were imaged for GFP (green) and for Llgl1/2 (red). x-y projections of three optical z slices spanning only 1.2 um of the apical cell regions are shown. The entire optical cross-sections of these cells along the white lines are at the bottom. Bars, 10 um. Dashed boxes show regions of higher magnifications (right). Note that some GFP and Llgl1/2 clusters are colocalized (arrowheads). (c) PCC values between green (GFP) and red (Llgl1/2) fluorescence of the random optical z slices of the images shown in b. (d) Western blot of the anti-GFP precipitates obtained from the confluent cultures of the control DLD cells and DLD20-2 cells expressing sLUR-517GFP mutants (marked as in Fig. 8, c and d ) probed for GFP and Llgl1/2.

LLGL1 Antibody — Figure 6. DLD1 cells deficient for Llgl1/2 exhibit only mild polarity defects. (a) Western blotting of total cell lysates of DLDcas9 cells (DLD), their Llgl2-deficient clone (Llgl2-KO), and the clone of the latter clone obtained after Llgl1 knockout (Llgl1/2-KO). The lysates were probed with antibodies indicated at the bottom. Note that the anti-Lgl1 antibody used in our study (ab18302) cross-reacts with Llgl2. (b) Phase contrast of the Llgl2- and Llgl1/2-deficient colonies (Llgl2-KO and Llgl1/2-KO). Arrows indicate the sites of clear multilayered organization. (c and d) Projections of all x-y optical slices of Llgl2- and Llgl1/2-deficient cells stained for E-cad (green) and CGN (red). The optical z-cross-sections of these images at the same magnification are shown at the bottom. Bars, 10 um. (e and f) The confluent 3-d-old cultures of Llgl2-KO (e) and Llgl1/2-KO (f) cells were stained with anti-cingulin antibody (cingulin, red) and with antibodies against polarity proteins (green): hScrib (Scrib), Pals1, Par6B (Par6), and aPKCzeta (aPKC). Bars, 10 um. Separate and merged Z stacks for each antibody staining at the same magnification are provided in the right panel. {KO}

Product Details

PA5-54637

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:200-1:500	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human LLGL1 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.4 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2643396

Product Specific Information

Immunogen sequence: FERFSLSARN ITEPLCSLDI NWPRDATQAS YRIRESPKLS QANGTPSILL APQSLDGSPD PAHSMGPDTP EPPEAALSPM SIDSATSADT TLDTTGDVTV EDVKDFLGSS EESEKNLRNL AEDE

Highest antigen sequence identity to the following orthologs: Mouse - 76%, Rat - 72%.

Target Information

LLGL1 is a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene for LLGL1 is located within the Smith-Magenis syndrome region on chromosome 17.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Scribble, Erbin, and Lano redundantly regulate epithelial polarity and apical adhesion complex.

The Journal of cell biology

Choi J,Troyanovsky RB,Indra I,Mitchell BJ,Troyanovsky SM

Published figure using LLGL1 polyclonal antibody (Product # PA5-54637) in Immunocytochemistry

Mon Jul 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: DLG4; Hugl protein; Hugl-1; Human homolog to the D-lgl gene protein; lethal giant larvae homolog 1; lethal giant larvae homolog 1, scribble cell polarity complex component; Lethal(2) giant larvae protein homolog 1; LLGL

Gene Aliases: DLG4; HUGL; HUGL-1; HUGL1; Lgl1; LLGL; LLGL1; Mgl1

UniProt ID: (Human) Q15334

Entrez Gene ID: (Human) 3996

Molecular Function: membrane trafficking regulatory protein

Function(s)

protein complex assembly exocytosis Golgi to plasma membrane transport axonogenesis regulation of Notch signaling pathway regulation of exocytosis neurogenesis cortical actin cytoskeleton organization regulation of establishment or maintenance of cell polarity positive regulation of GTPase activity regulation of protein secretion establishment of spindle orientation

Process(es)

GTPase activator activity structural molecule activity protein binding Rab GTPase binding protein kinase binding syntaxin binding

LLGL1 Polyclonal Antibody

Product Details

PA5-54637

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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