This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details
PA1-074 detects ERGIC-53 in rat, mouse and human samples.
PA1-074 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a ~57.5 kDa protein representing ERGIC-53 in rat brain samples.
The PA1-074 immunogen is a synthetic peptide corresponding to residues F(159) D S F D N D G K K N N P A I(173) of rat ERGIC-53. This sequence is conserved in mouse and human. The PA1-074 immunizing peptide (Cat. # PEP-277) is available for use in neutralization and control experiments.
The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: endoplasmic reticulum-golgi intermediate compartment protein 53; ER-Golgi intermediate compartment 53 kDa protein; Gp58; Intracellular mannose-specific lectin MR60; Lectin mannose-binding 1; lectin, mannose-binding, 1; p58; Protein ERGIC-53
Gene Aliases: 2610020P13Rik; AI326273; AU043785; C730041J05; ERGIC-53; ERGIC53; F5F8D; FMFD1; gp58; LMAN1; MCFD1; MR60; P58
Molecular Function: membrane traffic protein