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Immunofluorescent analysis of LONRF2 showing staining in the mitochondria of SK-N-SH cells. SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a LONRF2 polyclonal antibody (Product # PA5-35914) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 511 of LONRF2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
LONRF2 contains one Lon domain, one RING type zinc finger and six TPR repeats. The function of LONRF2 is unknown. There are two named isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
LON peptidase N-terminal domain and ring finger 2; neuroblastoma apoptosis-related protease; RING finger protein 192; RNF192