Invitrogen

LOX Polyclonal Antibody

LOX Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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LOX Antibody (PA1-16953) in IHC (P)

Immunohistochemical analysis of LOX in mouse stomach. Samples were incubated in LOX polyclonal antibody (Product # PA1-16953).

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LOX Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of LOX was observed in Mouse Heart (Lane 1) and Mouse Kidney (Lane 2) that express LOX protein, in comparison to, Mouse Spleen that doesnt express LOX protein using Anti-LOX Rabbit Polyclonal Antibody (Product # PA1-16953) in Western Blot. {RE}

Product Details

PA1-16953

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-4 µg/mL

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:250

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide between amino acids 200-300 of the human LOX protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_794775

Product Specific Information

PA1-16953 is specific for the ~58 kDa LOX protein.

This is a highly glycosylated protein and therefore, other bands may be seen at ~32 kDa (non-glycosylated form) 50 kDa and 58 kDa (glycosylated forms).

Suggested positive control: human and mouse kidney lysate, antigen standard for LOX (transient overexpression lysate), human kidney protein, mouse kidney protein.

Target Information

Lysyl oxidase (LOX), a copper-containing amine oxidase, belongs to a heterogeneous family of enzymes that oxidize primary amine substrates to reactive aldehydes. It plays a vital role in the formation and repair of the extra cellular matrix. As well, LOX is a multifunctional enzyme having diverse biological functions such as developmental regulation, tumor suppression, cell motility and cellular senescence. The secreted form of LOX is responsible for the invasive properties of hypoxic human cancer cells. Thus, it is essential for hypoxia-induced metastasis and is a good therapeutic target for preventing and treating metastases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neonatal exposure to hypoxia induces early arterial stiffening via activation of lysyl oxidases.

Physiological reports

Steppan J,Nandakumar K,Wang H,Jang R,Smith L,Kang S,Savage W,Bauer M,Choi R,Brady T,Wodu BP,Scafidi S,Scafidi J,Santhanam L

PA1-16953 was used in Western Blotting to investigate the impact of neonatal hypoxia on arterial stiffness and cardiovascular health, revealing that exposure to hypoxia during early life leads to significantly increased arterial stiffness in adulthood, potentially contributing to a higher risk of hypertension, with aberrant expression of lysyl oxidases (LOXs) and LOXL2 playing a crucial role in this process.

Sat Apr 01 00:00:00 UTC 2023

Neonatal exposure to hypoxia induces early arterial stiffening via activation of lysyl oxidases.

Physiological reports

Steppan J,Nandakumar K,Wang H,Jang R,Smith L,Kang S,Savage W,Bauer M,Choi R,Brady T,Wodu BP,Scafidi S,Scafidi J,Santhanam L

Sat Apr 01 00:00:00 UTC 2023

DuCLOX-2/5 Inhibition Attenuates Inflammatory Response and Induces Mitochondrial Apoptosis for Mammary Gland Chemoprevention.

Frontiers in pharmacology

Gautam S,Rawat AK,Sammi SR,Roy S,Singh M,Devi U,Yadav RK,Singh L,Rawat JK,Ansari MN,Saeedan AS,Kumar D,Pandey R,Kaithwas G

Published figure using LOX polyclonal antibody (Product # PA1-16953) in Western Blot

Thu Oct 01 00:00:00 UTC 2020

Defective signaling, osteoblastogenesis and bone remodeling in a mouse model of connexin 43 C-terminal truncation.

Journal of cell science

Moorer MC,Hebert C,Tomlinson RE,Iyer SR,Chason M,Stains JP

Published figure using LOX polyclonal antibody (Product # PA1-16953) in Western Blot

Wed Feb 01 00:00:00 UTC 2017

Elevated ischaemia-associated lysyl oxidase activity in delayed graft failure 6-12 months after renal transplantation.

Experimental physiology

Chen Z,Li Y,Xu H,Ma F,Li J,Zhao L,Xu Y

Published figure using LOX polyclonal antibody (Product # PA1-16953) in Western Blot

Wed Feb 01 00:00:00 UTC 2017

The Nox1/4 Dual Inhibitor GKT137831 or Nox4 Knockdown Inhibits Angiotensin-II-Induced Adult Mouse Cardiac Fibroblast Proliferation and Migration. AT1 Physically Associates With Nox4.

Journal of cellular physiology

Somanna NK,Valente AJ,Krenz M,Fay WP,Delafontaine P,Chandrasekar B

Published figure using LOX polyclonal antibody (Product # PA1-16953) in Western Blot

Sun May 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Lysyl oxidase; Lysyl oxidase (an H-rev gene with its expression down-regulated in HRAS-transformed rat 208F fibroblasts); MGC105112; Protein-lysine 6-oxidase; Ras excision protein; ras recision

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Gene Aliases: AI893619; H-rev142; LOX; Rrg; Rrg1; TSC-160

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UniProt ID: (Human) P28300, (Mouse) P28301

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Entrez Gene ID: (Pig) 100525278, (Human) 4015, (Rat) 24914, (Mouse) 16948

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Function(s)

protein-lysine 6-oxidase activity copper ion binding collagen binding protein binding oxidoreductase activity oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor metal ion binding oxidase

Process(es)

peptidyl-lysine oxidation blood vessel morphogenesis cellular protein modification process heart development extracellular matrix organization collagen fibril organization lung development aorta development wound healing response to drug elastic fiber assembly response to steroid hormone oxidation-reduction process blood vessel development response to hormone

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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LOX Polyclonal Antibody

LOX Antibody (PA1-16953) in IHC (P)

Product Details

PA1-16953

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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