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Invitrogen
LOX Polyclonal Antibody
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FIGURE: 1 / 7
LOX Antibody (PA1-16955) in ICC/IF
Immunocytochemistry analysis of LOX in HeLa cells. Samples were incubated with LOX polyclonal antibody (Product # PA1-16955) using a dilution of 1:100 dilution overnight at 4°C followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. Alpha tubulin (DM1A) was used as a co-stain at a 1:1,000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue... View More
Product Details
PA1-16955
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Pig,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Cocktail of two synthetic peptides between amino acids 300-350 and amino acids 200-300 of the human LOX protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is 92% homologous to chicken, zebrafish, and xenopus.
Target Information
Lysyl oxidase (LOX), a copper-containing amine oxidase, belongs to a heterogeneous family of enzymes that oxidize primary amine substrates to reactive aldehydes. It plays a vital role in the formation and repair of the extra cellular matrix. As well, LOX is a multifunctional enzyme having diverse biological functions such as developmental regulation, tumor suppression, cell motility and cellular senescence. The secreted form of LOX is responsible for the invasive properties of hypoxic human cancer cells. Thus, it is essential for hypoxia-induced metastasis and is a good therapeutic target for preventing and treating metastases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cross-linking enzyme; Lysyl oxidase; Lysyl oxidase (an H-rev gene with its expression down-regulated in HRAS-transformed rat 208F fibroblasts); MGC105112; Protein-lysine 6-oxidase; Ras excision protein; ras recision
Gene Aliases: AI893619; H-rev142; LOX; Rrg; Rrg1; TSC-160
UniProt ID: (Human) P28300, (Mouse) P28301
Entrez Gene ID: (Pig) 100525278, (Bovine) 280841, (Human) 4015, (Rat) 24914, (Mouse) 16948
peptidyl-lysine oxidation
blood vessel morphogenesis
cellular protein modification process
heart development
extracellular matrix organization
collagen fibril organization
lung development
aorta development
wound healing
response to drug
elastic fiber assembly
response to steroid hormone
oxidation-reduction process
blood vessel development
response to hormone
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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