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Agrisera
LOX/Lipoxygenase Polyclonal Antibody
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FIGURE: 1 / 1
LOX/Lipoxygenase Antibody (AS06-128) in WB
Samples of Arabidopsis thaliana (2), Olea europaea (3), Lilium Longiflorum (4), Lupinus luteus (5) were ground in liquid nitrogen to a very fine powder using a mortar and pestle and resuspended in 1.5 mL of extraction buffer (4% SDS, 2% 2-mercaptoethanol, 2 m M PMSF, 100 m M Tris-HCl p H 8.5). The samples were incubated for 3 min at 80°C. Protein suspensions were clarified by centrifugation at 13,500 g for 10 min at room temperature and the resulting supernatants were used. Total proteins (25 µg per sample) were separated by SDS-PAGE on Cr... View More
Product Details
AS06-128
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Native lipoxygenase, type I-B, purified from Glycine max.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Glycine max, Lilium longiflorum, Lupinus luteus, Musa acuminate, Musa paradisiaca L., Nicotiana benthamiana, Olea europaea, Oryza sativa
Target Information
Lysyl oxidase (LOX), a copper-containing amine oxidase, belongs to a heterogeneous family of enzymes that oxidize primary amine substrates to reactive aldehydes. It plays a vital role in the formation and repair of the extra cellular matrix. As well, LOX is a multifunctional enzyme having diverse biological functions such as developmental regulation, tumor suppression, cell motility and cellular senescence. The secreted form of LOX is responsible for the invasive properties of hypoxic human cancer cells. Thus, it is essential for hypoxia-induced metastasis and is a good therapeutic target for preventing and treating metastases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: expressed during ripening fruit; loxC; loxc homologue; MGC105112; TomloxC
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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