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Invitrogen
LRAT Polyclonal Antibody
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FIGURE: 1 / 3
LRAT Antibody (PA5-99243) in ICC/IF
Immunofluorescent analysis of LRAT in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and LRAT polyclonal antibody (Product # PA5-99243) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-99243
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human LRAT(Accession O95237), corresponding to amino acid residues Y118-C168.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total LRAT.
Target Information
Lecithin retinol acyltransferase (LRAT) is a membrane bound enzyme that catalyzes the transfer of an acyl group from the sn-1 position of lecithin to vitamin A which generates all-trans-retinyl esters (tREs) in the liver, some extrahepatic tissues, such as the lung, and retinal pigmented epithelium. LRAT can also exchange palmitoyl groups between RPE65, a tRE binding protein essential for vision, and tREs, which is important for the operation of the visual pathway. LRAT is essential for the dietary mobilization, transport, and storage of vitamin A as well as the synthesis of the visual pigment chromophore. LRAT monomers interact in membranes to form homodimers through disulfide bond formation. A loss of LRAT correlates with an early onset severe retinal dystrophy and severe retinyl ester deprivation, while a reduction in LRAT expression may be associated with invasive bladder cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Lecithin retinol acyltransferase; lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase); Phosphatidylcholine--retinol O-acyltransferase; Phosphatidylcholine-retinol-O-acyltransferase; unnamed protein product
Gene Aliases: 1300010A18Rik; AI449251; LCA14; LRAT
UniProt ID: (Human) O95237, (Mouse) Q9JI60, (Rat) Q9JI61
Entrez Gene ID: (Human) 9227, (Mouse) 79235, (Rat) 64047
retinoid metabolic process
lipid metabolic process
vitamin A metabolic process
visual perception
response to bacterium
lipid storage
positive regulation of lipid transport
response to retinoic acid
response to vitamin A
retinol metabolic process
cellular response to leukemia inhibitory factor
1,2-diacyl-sn-glycero-3-phosphocholine metabolic process
embryo development
response to stimulus
retinoic acid metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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