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Immunofluorescent analysis of LRP130 showing staining in the mitochondria of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using a LRP130 polyclonal antibody (Product # PA5-30357) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 244 and 500 of Human LRP130|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30357 targets LRP130 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-30357 immunogen is recombinant fragment corresponding to a region within amino acids 244 and 500 of Human LRP130.
This gene encodes a protein that is leucine-rich and is thought to play a role in regulating the interaction of the cytoskeleton with a variety of cellular processes. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome. Transcripts ranging in size from 4.8 to 7.0 kb which result from alternative polyadenylation have been reported for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
130 kDa leucine-rich protein; CLONE-23970; leucine-rich pentatricopeptide repeat containing; leucine-rich PPR motif-containing protein; leucine-rich PPR-motif containing; LRP 130; LRP130; LSFC; mitochondrial; mitochondrial leucine-rich PPR motif-containing protein
CLONE-23970; GP130; LRP130; LRPPRC; LSFC