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LRP2 Polyclonal Antibody

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LRP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 19700-1-AP ( LRP2-Specific Antibody) at dilution of 1:200 (under 40x lens).

LRP2 Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 19700-1-AP (LRP2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

LRP2 Antibody in Flow Cytometry (Flow) — 1X10^6 HEK-293 cells were stained with 0.2ug LRP2-Specific antibody (19700-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Product Details

19700-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated

Form

Liquid

Concentration

0.32 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody is specific to LRP2 and detects endogenous LRP2 with an apparent molecular weight 280 kDa, which is smaller than the predicted molecular weight (522 kDa) and probably represent a proteolytic fragment of the large glycoprotein.

Target Information

The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TMBIM6 promotes diabetic tubular epithelial cell survival and albumin endocytosis by inhibiting the endoplasmic reticulum stress sensor, IRE1α.

Molecular biology reports

Xie H,Shi Y,Zhou Y,Liu H

Sat Oct 01 00:00:00 EDT 2022

TMBIM6 promotes diabetic tubular epithelial cell survival and albumin endocytosis by inhibiting the endoplasmic reticulum stress sensor, IRE1α.

Molecular biology reports

Xie H,Shi Y,Zhou Y,Liu H

Sat Oct 01 00:00:00 EDT 2022

TMBIM6 promotes diabetic tubular epithelial cell survival and albumin endocytosis by inhibiting the endoplasmic reticulum stress sensor, IRE1α.

Molecular biology reports

Xie H,Shi Y,Zhou Y,Liu H

Sat Oct 01 00:00:00 EDT 2022

TMBIM6 promotes diabetic tubular epithelial cell survival and albumin endocytosis by inhibiting the endoplasmic reticulum stress sensor, IRE1α.

Molecular biology reports

Xie H,Shi Y,Zhou Y,Liu H

Sat Oct 01 00:00:00 EDT 2022

Effects of elevation of ANP and its deficiency on cardiorenal function.

JCI insight

Ilatovskaya DV,Levchenko V,Winsor K,Blass GR,Spires DR,Sarsenova E,Polina I,Zietara A,Paterson M,Kriegel AJ,Staruschenko A

Mon May 09 00:00:00 EDT 2022

Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.

EMBO reports

Chen Y,Wu X,Zhang J,Pan G,Wang X,Guo X,Wang J,Cui X,Gao H,Cheng M,Yang J,Zhang C,Jiang F

Thu Feb 03 00:00:00 EST 2022

Autophagy gene ATG7 regulates albumin transcytosis in renal tubule epithelial cells.

American journal of physiology. Renal physiology

Uchida Y,Torisu K,Ueki K,Tsuruya K,Nakano T,Kitazono T

Mon Nov 01 00:00:00 EDT 2021

Generation of a homozygous LRP2 knockout human embryonic stem cell line (FDCHDPe010-A-56) by CRISPR/Cas9 system.

Stem cell research

You J,Cheng Y,Yang XJ,Chen L

Sat May 01 00:00:00 EDT 2021

Sodium-glucose cotransporter 2 inhibition attenuates protein overload in renal proximal tubule via suppression of megalin O-GlcNacylation in progressive diabetic nephropathy.

Metabolism: clinical and experimental

Otomo H,Nara M,Kato S,Shimizu T,Suganuma Y,Sato T,Morii T,Yamada Y,Fujita H

Tue Dec 01 00:00:00 EST 2020

Sodium-glucose cotransporter 2 inhibition attenuates protein overload in renal proximal tubule via suppression of megalin O-GlcNacylation in progressive diabetic nephropathy.

Metabolism: clinical and experimental

Otomo H,Nara M,Kato S,Shimizu T,Suganuma Y,Sato T,Morii T,Yamada Y,Fujita H

Tue Dec 01 00:00:00 EST 2020

Kidney targeted delivery of asiatic acid using a FITC labeled renal tubular-targeting peptide modified PLGA-PEG system.

International journal of pharmaceutics

He J,Chen H,Zhou W,Chen M,Yao Y,Zhang Z,Tan N

Tue Jun 30 00:00:00 EDT 2020

Evidence for a regulated Ca²⁺ entry in proximal tubular cells and its implication in calcium stone formation.

Journal of cell science

Ibeh CL,Yiu AJ,Kanaras YL,Paal E,Birnbaumer L,Jose PA,Bandyopadhyay BC

Tue Apr 30 00:00:00 EDT 2019

Gelofusine Attenuates Tubulointerstitial Injury Induced by cRGD-Conjugated siRNA by Regulating the TLR3 Signaling Pathway.

Molecular therapy. Nucleic acids

Cen B,Liao W,Wang Z,Gao L,Wei Y,Huang W,He S,Wang W,Liu X,Pan X,Ji A

Fri Jun 01 00:00:00 EDT 2018

Gelofusine Attenuates Tubulointerstitial Injury Induced by cRGD-Conjugated siRNA by Regulating the TLR3 Signaling Pathway.

Molecular therapy. Nucleic acids

Cen B,Liao W,Wang Z,Gao L,Wei Y,Huang W,He S,Wang W,Liu X,Pan X,Ji A

Fri Jun 01 00:00:00 EDT 2018

Reduced proximal tubular expression of protein endocytic receptors in proteinuria is associated with urinary receptor shedding.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Fatah H,Benfaed N,Chana RS,Chunara MH,Barratt J,Baines RJ,Brunskill NJ

Fri Jun 01 00:00:00 EDT 2018

Reduced proximal tubular expression of protein endocytic receptors in proteinuria is associated with urinary receptor shedding.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Fatah H,Benfaed N,Chana RS,Chunara MH,Barratt J,Baines RJ,Brunskill NJ

Fri Jun 01 00:00:00 EDT 2018

Reduced proximal tubular expression of protein endocytic receptors in proteinuria is associated with urinary receptor shedding.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Fatah H,Benfaed N,Chana RS,Chunara MH,Barratt J,Baines RJ,Brunskill NJ

Fri Jun 01 00:00:00 EDT 2018

Reduced proximal tubular expression of protein endocytic receptors in proteinuria is associated with urinary receptor shedding.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Fatah H,Benfaed N,Chana RS,Chunara MH,Barratt J,Baines RJ,Brunskill NJ

Fri Jun 01 00:00:00 EDT 2018

TGF-β inhibits alveolar protein transport by promoting shedding, regulated intramembrane proteolysis, and transcriptional downregulation of megalin.

American journal of physiology. Lung cellular and molecular physiology

Mazzocchi LC,Vohwinkel CU,Mayer K,Herold S,Morty RE,Seeger W,Vadász I

Wed Nov 01 00:00:00 EDT 2017

TGF-β inhibits alveolar protein transport by promoting shedding, regulated intramembrane proteolysis, and transcriptional downregulation of megalin.

American journal of physiology. Lung cellular and molecular physiology

Mazzocchi LC,Vohwinkel CU,Mayer K,Herold S,Morty RE,Seeger W,Vadász I

Wed Nov 01 00:00:00 EDT 2017

Mouse early extra-embryonic lineages activate compensatory endocytosis in response to poor maternal nutrition.

Development (Cambridge, England)

Sun C,Velazquez MA,Marfy-Smith S,Sheth B,Cox A,Johnston DA,Smyth N,Fleming TP

Sat Mar 01 00:00:00 EST 2014

Mouse early extra-embryonic lineages activate compensatory endocytosis in response to poor maternal nutrition.

Development (Cambridge, England)

Sun C,Velazquez MA,Marfy-Smith S,Sheth B,Cox A,Johnston DA,Smyth N,Fleming TP

Sat Mar 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: calcium sensor protein; Glycoprotein 330; gp330; Heymann nephritis antigen homolog; low density lipoprotein receptor-related protein 2; Low-density lipoprotein receptor-related protein 2; LRP-2; Megalin

Gene Aliases: DBS; GP330; LRP2

UniProt ID: (Human) P98164

Entrez Gene ID: (Human) 4036

Molecular Function: transmembrane signal receptor

Function(s)

retinoid metabolic process ventricular septum development protein glycosylation lipid metabolic process endocytosis receptor-mediated endocytosis cell proliferation forebrain development aorta development vitamin D metabolic process lipoprotein transport coronary vasculature development

Process(es)

low-density lipoprotein receptor activity calcium ion binding protein binding SH3 domain binding lipoprotein transporter activity

LRP2 Polyclonal Antibody

Product Details

19700-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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