Immunofluorescent staining of LRP5-transfected 293T cells using Rb anti-LRP5 (Product # 36-5400). Image courtesy of Heekyung Chung, PhD, and Anthony Brown, PhD, Weill Medical College of Cornell University, NY.

LRP5 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

LRP5 Antibody — FIG. 5. Dkk1 treatment and LRP5/LRP6 siRNA stimulated neurite outgrowth. (A) Wnt-1 and Wnt-3a CM promoted beta-catenin stabilization in TC-32 cells. Stabilization of free beta-catenin was analyzed by GST-E-cadherin pull-down assay. Immunoblot (IB) analyses of beta-catenin pelleted with GST-E-cadherin and of beta-catenin and HSP70 in whole-cell lysates are indicated. -, absence of; +, presence of. (B) Dkk1 blocked Wnt-3a-induced beta-catenin stabilization in TC-32 cells. Cells were serum starved overnight and pretreated with Dkk1 for 30 min at 37degC in a CO 2 incubator. Wnt-3a CM or control L (Ctl.) CM were then added, and cells were further incubated for 3 h, followed by GST-E-cadherin pull-down assay. (C) Dkk1 induced neurite outgrowth. TC-32 cells plated on coverslips were pretreated with Dkk1 as indicated and subsequently incubated with Wnt-3a or control medium for 3 h. Then cells were fixed and stained with Alexa Fluor 568 phalloidin. Scale bars, 20 mum. (D) Quantitative analysis of the effect of Dkk1, Wnt-3a, and cotreatment with Dkk1 and Wnt-3a on neurite outgrowth. The percentage of cells having neurites was determined for each treatment group. The stimulation of neurite outgrowth for each group was defined as the ratio of this percentage to the percentage observed for the negative control. The results are means +- standard deviations (error bars) from three independent experiments. ***, P < 0.001. (E) Knockdown of LRP5 and LRP6 in TC-32 cells. Cells were transfected {KD}

Immunogen image

Immunogen Image LRP5 Polyclonal Antibody

Product Details

36-5400

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of the human LRP5 protein. This intracellular region is identical to the corresponding mouse and rat LRP5 amino acid sequences.

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533267

Target Information

LDL related proteins, LRP 5 and LRP 6, are members of the LDL receptor superfamily, which were found to be involved in the canonical Wnt signaling pathway. Mammalian LRP 6 was shown to bind to Wnt 1 and enhance Wnt-induced developmental processes in Xenopus embryos. Mice lacking LRP 6 exhibited developmental defects that are similar to those caused by deficiencies in various Wnt proteins. Recent work also shows that Wnt proteins induce the binding of LRP 5 to Axin, leading Axin degradation and beta catenin stabilization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HGNC:6697; low density lipoprotein receptor-related protein 5; low density lipoprotein receptor-related protein 7; Low-density lipoprotein receptor-related protein 5; Low-density lipoprotein receptor-related protein 7; LRP-5; LRP-7; LRP5

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Gene Aliases: BMND1; EVR1; EVR4; HBM; LR3; LRP-5; LRP-7; LRP5; LRP7; mKIAA4142; OPPG; OPS; OPTA1; PCLD4; VBCH2

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UniProt ID: (Human) O75197, (Mouse) Q91VN0

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Entrez Gene ID: (Human) 4041, (Mouse) 16973

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Function(s)

protein binding coreceptor activity Wnt-protein binding toxin transporter activity Wnt-activated receptor activity transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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LRP5 Polyclonal Antibody

LRP5 Antibody (36-5400) in ICC/IF

Product Details

36-5400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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