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          • Primary Antibodies ›
          • LRP6 Antibodies

          Invitrogen

          LRP6 Polyclonal Antibody

          Advanced Verification
          View all (30) LRP6 antibodies

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          Cite LRP6 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          • Advanced Verification (1)
          LRP6 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          LRP6 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          LRP6 Antibody (PA5-101047) in ICC/IF

          Immunofluorescence analysis of LRP6 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with LRP6 Polyclonal Antibody (Product # PA5-101047) at 1:100 dilution in 0.1% BSA, incubated at 4 degrees celsius overnight, and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A3... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          LRP6 Antibody in Immunocytochemistry (ICC/IF)
          LRP6 Antibody in Western Blot (WB)
          LRP6 Antibody in Western Blot (WB)
          LRP6 Antibody
          LRP6 Polyclonal Antibody

          Product Details

          PA5-101047

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:3,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:100
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human LRP6(Accession O75581), corresponding to amino acid residues G1454-Y1504.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2815876

          Product Specific Information

          Antibody detects endogenous levels of total LRP6.

          Target Information

          LRP6 is essential for the Wnt/beta catenin signaling pathway, probably by acting as a coreceptor together with Frizzled for Wnt. It is a specific, high-affinity receptor for DKK1 and DKK2, but not DKK3. The interaction with DKK1 blocks LRP6-mediated Wnt/beta catenin signaling via LRP6 removal via Kremen proteins-mediated endocytosis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: crooked; FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; low density lipoprotein-related protein 6; low density lipoprotein-related protein 6; Low-density lipoprotein receptor-related protein 6; lpr6; LRP-6; transmembrane protein; unnamed protein product

          View more View less

          Gene Aliases: ADCAD2; C030016K15Rik; Cd; Gw; LRP6; ska26; ska; skax26; STHAG7

          View more View less

          UniProt ID: (Human) O75581, (Mouse) O88572

          View more View less

          Entrez Gene ID: (Human) 4040, (Rat) 312781, (Mouse) 16974

          View more View less

          Function(s)
          low-density lipoprotein receptor activity receptor binding frizzled binding protein binding coreceptor activity Wnt-protein binding kinase inhibitor activity toxin transporter activity protein serine/threonine kinase inhibitor activity identical protein binding protein homodimerization activity Wnt-activated receptor activity molecular_function apolipoprotein binding coreceptor activity involved in Wnt signaling pathway apolipoprotein
          Process(es)
          regulation of transcription, DNA-templated endocytosis positive regulation of cytosolic calcium ion concentration chemical synaptic transmission nervous system development tissue development neural crest formation neural crest cell differentiation Wnt signaling pathway vesicle-mediated transport midbrain development negative regulation of smooth muscle cell apoptotic process regulation of apoptotic process chordate embryonic development response to peptide hormone positive regulation of cell cycle positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter anatomical structure formation involved in morphogenesis transmembrane transport canonical Wnt signaling pathway cellular response to cholesterol dopaminergic neuron differentiation protein localization to plasma membrane cell-cell adhesion midbrain dopaminergic neuron differentiation gastrulation with mouth forming second somitogenesis neural tube closure negative regulation of protein phosphorylation heart looping morphogenesis of an epithelium positive regulation of mesenchymal cell proliferation pericardium morphogenesis axis elongation negative regulation of protein kinase activity embryonic pattern specification dorsal/ventral axis specification anterior/posterior pattern specification single organismal cell-cell adhesion cerebellum morphogenesis thalamus development cerebral cortex cell migration forebrain radial glial cell differentiation forebrain generation of neurons Wnt signaling pathway involved in forebrain neuroblast division neural tube development formation of radial glial scaffolds cerebral cortex development negative regulation of Wnt signaling pathway regulation of ossification embryonic limb morphogenesis forebrain development midbrain-hindbrain boundary development limb morphogenesis embryonic forelimb morphogenesis embryonic hindlimb morphogenesis external genitalia morphogenesis post-anal tail morphogenesis cell migration involved in gastrulation regulation of cell proliferation odontogenesis of dentin-containing tooth embryonic digit morphogenesis positive regulation of apoptotic process Wnt signaling pathway involved in dorsal/ventral axis specification regulation of fat cell differentiation negative regulation of fat cell differentiation positive regulation of ossification positive regulation of bone resorption bone remodeling embryonic camera-type eye morphogenesis generation of neurons skeletal system morphogenesis negative regulation of epithelial cell proliferation positive regulation of sequence-specific DNA binding transcription factor activity response to folic acid palate development convergent extension retina morphogenesis in camera-type eye embryonic retina morphogenesis in camera-type eye regulation of cell development face morphogenesis bone morphogenesis branching involved in mammary gland duct morphogenesis trachea cartilage morphogenesis mammary placode formation mammary gland duct morphogenesis canonical Wnt signaling pathway involved in cardiac neural crest cell differentiation involved in heart development canonical Wnt signaling pathway involved in positive regulation of cardiac outflow tract cell proliferation negative regulation of protein serine/threonine kinase activity primitive streak formation receptor-mediated endocytosis of low-density lipoprotein particle involved in cholesterol transport Wnt signaling pathway involved in somitogenesis axis elongation involved in somitogenesis positive regulation of canonical Wnt signaling pathway toxin transport negative regulation of non-canonical Wnt signaling pathway positive regulation of Wnt signaling pathway involved in dorsal/ventral axis specification negative regulation of planar cell polarity pathway involved in ventricular septum morphogenesis negative regulation of planar cell polarity pathway involved in cardiac muscle tissue morphogenesis negative regulation of planar cell polarity pathway involved in cardiac right atrium morphogenesis negative regulation of planar cell polarity pathway involved in outflow tract morphogenesis negative regulation of planar cell polarity pathway involved in pericardium morphogenesis negative regulation of planar cell polarity pathway involved in neural tube closure receptor-mediated endocytosis multicellular organism development
          It has to be done as per old AB suggested Products section.

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