Invitrogen

LRP6 Polyclonal Antibody

View all (21) LRP6 antibodies

Questions & Answers

Questions & Answers

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LRP6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of LRP6 in human Melanoma tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115523) at a dilution of 1:100. Secondary staining was applied with HRP conjugated goat anti-rabbit.

LRP6 Antibody in Western Blot (WB) — Western blot analysis of LRP6 in COLO205 whole lysates. Samples were incubated with polyclonal antibody (Product # PA5-115523).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human LRP6, corresponding to a region within C-terminal amino acids.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 150mM NaCl, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

LRP6 is essential for the Wnt/beta catenin signaling pathway, probably by acting as a coreceptor together with Frizzled for Wnt. It is a specific, high-affinity receptor for DKK1 and DKK2, but not DKK3. The interaction with DKK1 blocks LRP6-mediated Wnt/beta catenin signaling via LRP6 removal via Kremen proteins-mediated endocytosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; Low-density lipoprotein receptor-related protein 6; lpr6; LRP-6

Gene Aliases: ADCAD2; LRP6; STHAG7

UniProt ID: (Human) O75581

Entrez Gene ID: (Human) 4040

Molecular Function: apolipoprotein

neural tube closure negative regulation of protein phosphorylation pericardium morphogenesis negative regulation of protein kinase activity positive regulation of cytosolic calcium ion concentration chemical synaptic transmission embryonic pattern specification neural crest formation neural crest cell differentiation Wnt signaling pathway single organismal cell-cell adhesion cerebellum morphogenesis thalamus development cerebral cortex development midbrain-hindbrain boundary development negative regulation of smooth muscle cell apoptotic process external genitalia morphogenesis odontogenesis of dentin-containing tooth response to peptide hormone Wnt signaling pathway involved in dorsal/ventral axis specification canonical Wnt signaling pathway involved in neural crest cell differentiation canonical Wnt signaling pathway involved in regulation of cell proliferation positive regulation of cell cycle positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of sequence-specific DNA binding transcription factor activity palate development convergent extension embryonic retina morphogenesis in camera-type eye canonical Wnt signaling pathway face morphogenesis trachea cartilage morphogenesis cellular response to cholesterol dopaminergic neuron differentiation negative regulation of protein serine/threonine kinase activity protein localization to plasma membrane primitive streak formation negative regulation of canonical Wnt signaling pathway receptor-mediated endocytosis of low-density lipoprotein particle involved in cholesterol transport Wnt signaling pathway involved in somitogenesis axis elongation involved in somitogenesis positive regulation of canonical Wnt signaling pathway toxin transport beta-catenin destruction complex disassembly midbrain dopaminergic neuron differentiation Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation positive regulation of Wnt signaling pathway involved in dorsal/ventral axis specification

Process(es)

low-density lipoprotein receptor activity receptor binding frizzled binding protein binding Wnt-protein binding kinase inhibitor activity toxin transporter activity apolipoprotein binding identical protein binding protein homodimerization activity Wnt-activated receptor activity coreceptor activity involved in Wnt signaling pathway coreceptor activity involved in canonical Wnt signaling pathway