Invitrogen

LRP6 Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (25) LRP6 antibodies

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LRP6 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of LRP6 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with LRP6 Polyclonal Antibody (Product # PA5-115523) at 1:100 dilution in 0.1% BSA, incubated at 4 degrees celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing endoplasmic reticulum localization. Panel e represents U937 cells that express LRP6 only at a very low level (as per various gene and protein expression databases). Panel f represents control cells with no primary antibody to assess the background. The images were captured at 60X magnification.

LRP6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of LRP6 in human Melanoma tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115523) at a dilution of 1:100. Secondary staining was applied with HRP conjugated goat anti-rabbit.

LRP6 Antibody in Western Blot (WB) — Western blot analysis of LRP6 in COLO205 whole lysates. Samples were incubated with polyclonal antibody (Product # PA5-115523).

LRP6 Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-LRP6 Polyclonal Antibody (Product # PA5-115523), shows a high level of expression in the A549 cell line as compared to the U937 cell line. {RE}

Product Details

PA5-115523

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:3,000	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:200	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human LRP6, corresponding to a region within C-terminal amino acids. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2900159

Target Information

LRP6 is essential for the Wnt/beta catenin signaling pathway, probably by acting as a coreceptor together with Frizzled for Wnt. It is a specific, high-affinity receptor for DKK1 and DKK2, but not DKK3. The interaction with DKK1 blocks LRP6-mediated Wnt/beta catenin signaling via LRP6 removal via Kremen proteins-mediated endocytosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; Low-density lipoprotein receptor-related protein 6; lpr6; LRP-6

Gene Aliases: ADCAD2; LRP6; STHAG7

UniProt ID: (Human) O75581

Entrez Gene ID: (Human) 4040

Molecular Function: apolipoprotein

neural tube closure negative regulation of protein phosphorylation pericardium morphogenesis negative regulation of protein kinase activity positive regulation of cytosolic calcium ion concentration chemical synaptic transmission embryonic pattern specification neural crest formation neural crest cell differentiation Wnt signaling pathway single organismal cell-cell adhesion cerebellum morphogenesis thalamus development cerebral cortex development midbrain-hindbrain boundary development negative regulation of smooth muscle cell apoptotic process external genitalia morphogenesis odontogenesis of dentin-containing tooth response to peptide hormone Wnt signaling pathway involved in dorsal/ventral axis specification canonical Wnt signaling pathway involved in neural crest cell differentiation canonical Wnt signaling pathway involved in regulation of cell proliferation positive regulation of cell cycle positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of sequence-specific DNA binding transcription factor activity palate development convergent extension embryonic retina morphogenesis in camera-type eye canonical Wnt signaling pathway face morphogenesis trachea cartilage morphogenesis cellular response to cholesterol dopaminergic neuron differentiation negative regulation of protein serine/threonine kinase activity protein localization to plasma membrane primitive streak formation negative regulation of canonical Wnt signaling pathway receptor-mediated endocytosis of low-density lipoprotein particle involved in cholesterol transport Wnt signaling pathway involved in somitogenesis axis elongation involved in somitogenesis positive regulation of canonical Wnt signaling pathway toxin transport beta-catenin destruction complex disassembly midbrain dopaminergic neuron differentiation Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation positive regulation of Wnt signaling pathway involved in dorsal/ventral axis specification

Process(es)

low-density lipoprotein receptor activity receptor binding frizzled binding protein binding Wnt-protein binding kinase inhibitor activity toxin transporter activity apolipoprotein binding identical protein binding protein homodimerization activity Wnt-activated receptor activity coreceptor activity involved in Wnt signaling pathway coreceptor activity involved in canonical Wnt signaling pathway