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LRPPRC Polyclonal Antibody

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LRPPRC Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using LRPPRC antibody 21175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

LRPPRC Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 21175-1-AP (LRPPRC antibody) at dilution of 1:100 (under 40x lens).

LRPPRC Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

LRPPRC Antibody in Immunoprecipitation (IP) — IP result of anti-LRPPRC (IP:21175-1-AP, 5ug; Detection:21175-1-AP 1:1000) with HeLa cells lysate 1200ug.

Product Details

21175-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:8,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LRPPRC Fusion Protein Ag15452 (1014-1394 aa encoded by BC050311) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: LWYEDEKHS LNSSSASTTE PDFQKDILIA CRLNQKKGAY DIFLNAKEQN IVFNAETYSN LIKLLMSEDY FTQAMEVKAF AETHIKGFTL NDAANSRLII TQVRRDYLKE AVTTLKTVLD QQQTPSRLAV TRVIQALAMK GDVENIEVVQ KMLNGLEDSI GLSKMVFINN IALAQIKNNN IDAAIENIEN MLTSENKVIE PQYFGLAYLF RKVIEEQLEP AVEKISIMAE RLANQFAIYK PVTDFFLQLV DAGKVDDARA LLQRCGAIAE QTPILLLFLL RNSRKQGKAS TVKSVLELIP ELNEKEEAYN SLMKSYVSEK DVTSAKALYE HLTAKNTKLD DLFLKRYASL LKYAGEPVPF IEPPESFEFY AQQLRKLREN SS (1014-1394 aa encoded by B C050311)

Target Information

This gene encodes a deadenylase that functions as the catalytic subunit of the polyadenylate binding protein dependent poly(A) nuclease complex. The encoded protein is a magnesium dependent 3' to 5' exoribonuclease that is involved in the degradation of cytoplasmic mRNAs. Alternate splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames.

Nature biotechnology

Liang XH,Shen W,Sun H,Migawa MT,Vickers TA,Crooke ST

Mon Aug 01 00:00:00 EDT 2016

Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells.

The EMBO journal

Ma Y,Yao N,Liu G,Dong L,Liu Y,Zhang M,Wang F,Wang B,Wei X,Dong H,Wang L,Ji S,Zhang J,Wang Y,Huang Y,Yu J

Tue Feb 03 00:00:00 EST 2015

Bioinformatics

Protein Aliases: 130 kDa leucine-rich protein; GP130; Leucine rich protein 157; leucine rich protein LRP130; leucine-rich pentatricopeptide repeat containing; Leucine-rich PPR motif-containing protein, mitochondrial; leucine-rich PPR-motif containing; leucine-rich protein 157; LRP 130; mitochondrial leucine-rich PPR motif-containing protein; rLRP157

Gene Aliases: 3110001K13Rik; C76645; CLONE-23970; GP130; LRP130; Lrp157; LRPPRC; LSFC

UniProt ID: (Human) P42704, (Rat) Q5SGE0, (Mouse) Q6PB66

Entrez Gene ID: (Human) 10128, (Rat) 313867, (Mouse) 72416

Function(s)

negative regulation of mitochondrial RNA catabolic process transcription, DNA-templated regulation of transcription, DNA-templated mitochondrion transport along microtubule mRNA transport regulation of mitochondrial translation biological_process transport

Process(es)

single-stranded DNA binding RNA binding protein binding microtubule binding ubiquitin protein ligase binding poly(A) RNA binding beta-tubulin binding actin filament binding molecular_function DNA binding

LRPPRC Polyclonal Antibody

Product Details

21175-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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