Invitrogen

LRRK2 Polyclonal Antibody

View all (18) LRRK2 antibodies

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LRRK2 Antibody in Western Blot (WB) — Western Blot analysis of LRRK2 was performed in rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes. Lane 1,2: LRRK2 Antibody (Product # PA5-111858) at a dilution of 1:200. Lane 3,4: LRRK2 Antibody preincubated with the negative control antigen.

LRRK2 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry analysis of LRRK2 in perfusion-fixed, frozen rat striatum and substantia nigra tissue sections using LRRK2 Antibody (Product # PA5-111858) at a dilution of 1:400, followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. A) LRKK2 staining (green) of striatum sections is detected in the cytoplasm of several striatal cells (arrows). B) Staining in the substantia nigra pars compacta shows LRKK2 staining (green) in nuclei and cytoplasm of several cells (arrows). Nuclei were stained with DAPI (blue).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

-

Immunohistochemistry (Frozen) (IHC (F))

1:400

-

Immunocytochemistry (ICC)

Assay-dependent

-

Immunofluorescence (IF)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide corresponding to amino acid residues 183-196 of mouse LRRK2 (N-terminus)

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

RRID

AB_2857267

Product Specific Information

Reconstitute antibody with 50 µL of double distilled water (DDW). The reconstituted solution can be stored at 4 degrees C for up to 1 week. For longer periods, small aliquots should be stored at -20 degrees C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Peptide sequence is: CKALHVLFER VSEE

Homology: Rat, human amino acid residues are identical.

Target Information

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: augmented in rheumatoid arthritis 17; BC 300-268; Dardarin; DKFZp434H2111; FLJ45829; leucine-rich repeat kinase 2; Leucine-rich repeat serine/threonine-protein kinase 2; LRR kinase 2; LRRK 2; LRRK-2

Gene Aliases: 4921513O20Rik; 9330188B09Rik; AURA17; AW561911; cI-46; D630001M17Rik; DARDARIN; Gm927; LRRK2; PARK8; RIPK7; ROCO2

UniProt ID: (Human) Q5S007, (Mouse) Q5S006

Entrez Gene ID: (Human) 120892, (Mouse) 66725, (Rat) 300160

Molecular Function: kinase non-receptor serine/threonine protein kinase non-receptor tyrosine protein kinase protein kinase transferase

MAPK cascade activation of MAPKK activity activation of MAPK activity negative regulation of protein phosphorylation positive regulation of protein phosphorylation protein phosphorylation endocytosis autophagy response to oxidative stress mitochondrion organization Golgi organization lysosome organization small GTPase mediated signal transduction neuromuscular junction development determination of adult lifespan cellular response to starvation regulation of autophagy positive regulation of autophagy regulation of protein kinase A signaling negative regulation of protein processing regulation of neuron maturation negative regulation of macroautophagy phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation calcium-mediated signaling olfactory bulb development tangential migration from the subventricular zone to the olfactory bulb positive regulation of protein ubiquitination negative regulation of protein binding positive regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process negative regulation of GTPase activity cellular response to oxidative stress cellular protein localization intracellular signal transduction regulation of kidney size exploration behavior locomotory exploration behavior regulation of lysosomal lumen pH regulation of locomotion regulation of membrane potential positive regulation of programmed cell death positive regulation of MAP kinase activity positive regulation of GTPase activity GTP metabolic process protein autophosphorylation intracellular distribution of mitochondria neuron projection morphogenesis regulation of calcium-mediated signaling mitochondrion localization regulation of mitochondrial depolarization regulation of synaptic transmission, glutamatergic canonical Wnt signaling pathway excitatory postsynaptic potential regulation of dopamine receptor signaling pathway positive regulation of dopamine receptor signaling pathway regulation of canonical Wnt signaling pathway regulation of dendritic spine morphogenesis protein localization to mitochondrion neuron death cellular response to manganese ion reactive oxygen species metabolic process regulation of mitochondrial fission positive regulation of canonical Wnt signaling pathway negative regulation of excitatory postsynaptic potential regulation of neuron death negative regulation of neuron death negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of protein autoubiquitination regulation of neuroblast proliferation regulation of synaptic vesicle transport negative regulation of late endosome to lysosome transport negative regulation of autophagosome assembly negative regulation of thioredoxin peroxidase activity by peptidyl-threonine phosphorylation negative regulation of hydrogen peroxide-induced cell death negative regulation of protein targeting to mitochondrion negative regulation of protein processing involved in protein targeting to mitochondrion cellular response to dopamine Wnt signalosome assembly regulation of retrograde transport, endosome to Golgi regulation of branching morphogenesis of a nerve regulation of synaptic vesicle exocytosis negative regulation of peroxidase activity cell differentiation synaptic vesicle recycling cellular response to organic cyclic compound

Process(es)

SNARE binding glycoprotein binding actin binding GTPase activity protein kinase activity protein serine/threonine kinase activity MAP kinase kinase activity GTPase activator activity protein binding ATP binding GTP binding microtubule binding tubulin binding kinase activity Rho GTPase binding syntaxin-1 binding receptor signaling complex scaffold activity clathrin binding GTP-dependent protein kinase activity peroxidase inhibitor activity co-receptor binding identical protein binding protein homodimerization activity ion channel binding protein kinase A binding beta-catenin destruction complex binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups molecular_function