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          • Primary Antibodies ›
          • LRRK2 Antibodies

          Invitrogen

          LRRK2 Polyclonal Antibody

          View all (66) LRRK2 antibodies

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          Cite LRRK2 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          LRRK2 Antibody in Immunocytochemistry (ICC/IF)
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          LRRK2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          LRRK2 Antibody (PA5-18319) in ICC/IF

          Immunocytochemical analysis of LRRK2 in A549 cells using a LRRK2 polyclonal antibody (Product #PA5-18319). A549 cells were permeabilized with 0.15% Triton. Cells were incubated with 10 µg/mL of primary antibody for one hour followed by an Alexa Fluor 488 secondary antibody at a concentration of 2 µg/mL. Nuclear and vesicle staining is shown in the image. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (2 µg/mL). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          LRRK2 Antibody in Immunocytochemistry (ICC/IF)
          LRRK2 Antibody in Immunocytochemistry (ICC/IF)
          LRRK2 Polyclonal Antibody

          Product Details

          PA5-18319

          Applications
          Tested Dilution
          Publications

          Immunocytochemistry (ICC/IF)

          10 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Goat / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide sequence (CELAEKMRRTSV) corresponding to the internal amino acids of LRRK2 (aa 2516-2526).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Amount

          100 µg

          Purification

          Ammonium sulfate precipitation

          Storage buffer

          TBS, pH 7.3, with 0.5% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10988665

          Product Specific Information

          This antibody is predicted to react with bovine based on sequence homology.

          This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

          Target Information

          LRRK2 is a multidomain protein that includes a kinase domain, a GTPase domain, and a leucine-rich repeat (LRR) domain. Mutations in LRRK2 have been associated with Parkinson's disease. LRRK2 is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: augmented in rheumatoid arthritis 17; BC 300-268; Dardarin; DKFZp434H2111; FLJ45829; Leucine-rich repeat serine/threonine-protein kinase 2; LRR kinase 2; LRRK 2; LRRK-2

          View more View less

          Gene Aliases: AURA17; DARDARIN; LRRK2; PARK8; RIPK7; ROCO2

          View more View less

          UniProt ID: (Human) Q5S007

          View more View less

          Entrez Gene ID: (Human) 120892

          View more View less

          Function(s)
          SNARE binding nucleotide binding magnesium ion binding actin binding GTPase activity protein kinase activity protein serine/threonine kinase activity JUN kinase kinase kinase activity MAP kinase kinase kinase activity GTPase activator activity protein binding ATP binding GTP binding microtubule binding tubulin binding kinase activity transferase activity hydrolase activity syntaxin-1 binding receptor signaling complex scaffold activity clathrin binding small GTPase binding GTP-dependent protein kinase activity peroxidase inhibitor activity co-receptor binding identical protein binding protein homodimerization activity ion channel binding protein kinase A binding protein serine kinase activity beta-catenin destruction complex binding
          Process(es)
          MAPK cascade protein phosphorylation protein import into nucleus endocytosis autophagy response to oxidative stress mitochondrion organization endoplasmic reticulum organization Golgi organization lysosome organization cell surface receptor signaling pathway JNK cascade Rho protein signal transduction spermatogenesis neuromuscular junction development protein localization determination of adult lifespan cellular response to starvation anatomical structure morphogenesis negative regulation of signal transduction regulation of gene expression regulation of autophagy positive regulation of autophagy negative regulation of protein processing negative regulation of neuron projection development regulation of neuron maturation vesicle-mediated transport negative regulation of macroautophagy calcium-mediated signaling striatum development olfactory bulb development tangential migration from the subventricular zone to the olfactory bulb cell differentiation regulation of proteolysis neuron differentiation regulation of cell projection organization positive regulation of protein ubiquitination regulation of protein stability positive regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of tumor necrosis factor production negative regulation of GTPase activity cellular response to oxidative stress cellular response to reactive oxygen species regulation of SNARE complex assembly intracellular signal transduction regulation of kidney size exploration behavior locomotory exploration behavior regulation of lysosomal lumen pH regulation of locomotion regulation of membrane potential positive regulation of programmed cell death positive regulation of MAPK cascade negative regulation of Notch signaling pathway GTP metabolic process protein autophosphorylation intracellular distribution of mitochondria animal organ development neuron projection morphogenesis modulation of synaptic transmission regulation of multicellular organismal process positive regulation of protein metabolic process mitochondrion localization regulation of mitochondrial depolarization regulation of synaptic transmission, glutamatergic canonical Wnt signaling pathway excitatory postsynaptic potential regulation of dopamine receptor signaling pathway positive regulation of dopamine receptor signaling pathway regulation of vesicle-mediated transport regulation of ER to Golgi vesicle-mediated transport regulation of canonical Wnt signaling pathway regulation of dendritic spine morphogenesis protein localization to mitochondrion protein localization to endoplasmic reticulum exit site cellular response to manganese ion regulation of mitochondrial fission positive regulation of canonical Wnt signaling pathway negative regulation of excitatory postsynaptic potential neuron projection arborization regulation of cAMP/PKA signal transduction regulation of synaptic vesicle endocytosis positive regulation of synaptic vesicle endocytosis positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of protein autoubiquitination regulation of intracellular signal transduction regulation of neuroblast proliferation regulation of synaptic vesicle transport negative regulation of late endosome to lysosome transport negative regulation of autophagosome assembly negative regulation of protein targeting to mitochondrion negative regulation of protein processing involved in protein targeting to mitochondrion cellular response to dopamine positive regulation of microglial cell activation cellular response to curcumin Wnt signalosome assembly regulation of retrograde transport, endosome to Golgi regulation of CAMKK-AMPK signaling cascade regulation of branching morphogenesis of a nerve regulation of synaptic vesicle exocytosis regulation of reactive oxygen species metabolic process
          It has to be done as per old AB suggested Products section.

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