Invitrogen

LRRTM1 Polyclonal Antibody

View all (19) LRRTM1 antibodies

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Datasheet

Protocols

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LRRTM1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of of LRRTM1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with LRRTM1 Polyclonal Antibody (Product # PA5-119942) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

LRRTM1 Antibody in Western Blot (WB) — Western blot analysis of LRRTM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. LRRTM1 Polyclonal Antibody (Product # PA5-119942) at 1:500 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate. Lane 2: N2A cell lysate. Lane 1: Rat brain tissue lysate.

LRRTM1 Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of LRRTM1 in SH-SY5Y cells using LRRTM1 Polyclonal Antibody (Product # PA5-119942) at 1 µg/mL (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at 4°C for 1 hour, the cells were stained with a Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1:1,000 dilution for 30 minutes at 4°C (dark incubation). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Product Details

PA5-119942

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:100	-
Flow Cytometry (Flow)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide within mouse LRRTM1 aa 320-360/522 (Extracellular) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	TBS, pH 7.4, with 50% glycerol
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2913514

Product Specific Information

Positive Control: SH-SY5Y cell lysate, N2A cell lysate, rat brain tissue lysate, N2A, SH-SY5Y.

Subcellular Location: Cell membrane, postsynaptic cell membrane.

Target Information

The Leucine-rich repeat transmembrane neuronal proteins (LRRTMs) are differentially expressed in the nervous system and were recently found to instruct presynaptic and mediate postsynaptic glutamatergic differentiation, with LRRTM1 and LRRTM2 most potent at inducing presynaptic differentiation. Each LRRTM protein is a type I transmembrane containing ten extracellular leucine-rich repeats and a short intracellular tail and has a developmentally regulated pattern distinct from all others. LRRTM1 is a maternally suppressed gene that is associated paternally with handedness and schizophrenia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: leucine-rich repeat transmembrane neuronal 1; leucine-rich repeat transmembrane neuronal 1 protein; Leucine-rich repeat transmembrane neuronal protein 1

Gene Aliases: 4632401D06Rik; AW125451; LRRTM1; UNQ675/PRO1309

UniProt ID: (Rat) D4A6D8, (Human) Q86UE6, (Mouse) Q8K377

Entrez Gene ID: (Rat) 679668, (Human) 347730, (Mouse) 74342

Molecular Function: transmembrane signal receptor