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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • LSM7 Antibodies

          Invitrogen

          LSM7 Polyclonal Antibody

          View all (5) LSM7 antibodies

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          Cite LSM7 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (19)
          LSM7 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          LSM7 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 19

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          LSM7 Antibody (PA5-143997) in ICC/IF

          Immunocytochemistry analysis of LSM7 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in LSM7 Polyclonal antibody (Product # PA5-143997) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate fo... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          LSM7 Antibody in Immunocytochemistry (ICC/IF)
          LSM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM7 Antibody in Western Blot (WB)
          LSM7 Antibody in Flow Cytometry (Flow)
          LSM7 Antibody in Flow Cytometry (Flow)
          LSM7 Polyclonal Antibody

          Product Details

          PA5-143997

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.25-0.5 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1-2 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived human LSM7 recombinant protein (Position: M1-A103).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          0.01mg sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3075211

          Product Specific Information

          Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

          Positive Control - WB: human HL-60 whole cell, human HEPG2 whole cell, human U937 whole cell, human HEK293 whole cell, human MCF-7 whole cell, human Jurkat whole cell, human Raji whole cell, rat brain tissue, mouse brain tissue. IHC: human bladder cancer tissue, mouse brain tissue, rat brain tissue, mouse brain tissue, rat brain tissue, rat cerebellum tissue, human melanoma tissue, human ovary cancer tissue, human appendicitis tissue, human bladder cancer tissue, human gastric cancer tissue, human renal carcinoma tissue, human skin cancer tissue, human tonsil tissue, mouse cerebellum tissue. ICC/IF: MCF-7 cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: LSM7 homolog, U6 small nuclear RNA associated; LSM7 U6 small nuclear RNA and mRNA degradation associated; U6 snRNA-associated Sm-like protein LSm7

          View more View less

          Gene Aliases: 0910001B06Rik; 1110033F18Rik; LSM7; YNL147W

          View more View less

          UniProt ID: (Human) Q9UK45, (Mouse) Q9CQQ8

          View more View less

          Entrez Gene ID: (Human) 51690, (Mouse) 66094, (Rat) 362829

          View more View less

          Function(s)
          RNA binding protein binding U6 snRNA binding protein heterodimerization activity RNA splicing factor
          Process(es)
          mRNA splicing, via spliceosome RNA processing exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay mRNA catabolic process
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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