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          • Primary Antibodies ›
          • LSM8 Antibodies

          Invitrogen

          LSM8 Polyclonal Antibody

          View all (4) LSM8 antibodies

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          Cite LSM8 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (14)
          LSM8 Antibody in Immunocytochemistry (ICC/IF)
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          LSM8 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

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          LSM8 Antibody (PA5-144015) in ICC/IF

          Immunocytochemistry analysis of LSM8 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in LSM8 Polyclonal antibody (Product # PA5-144015) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          LSM8 Antibody in Immunocytochemistry (ICC/IF)
          LSM8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LSM8 Antibody in Western Blot (WB)
          LSM8 Antibody in Flow Cytometry (Flow)
          LSM8 Antibody in Flow Cytometry (Flow)
          LSM8 Polyclonal Antibody

          Product Details

          PA5-144015

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.25 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1-2 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived human LSM8 recombinant protein (Position: M1-H96).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          0.01mg sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3075229

          Product Specific Information

          Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

          Positive Control - WB: human HELA whole cell, human HL-60 whole cell, human HEPG2 whole cell, human U937 whole cell, human Jurkat whole cell, human Raji whole cell, human THP-1 whole cell, human K562 whole cell, rat testis tissue, rat lung tissue, rat liver tissue, rat pancreas tissue, mouse testis tissue, mouse lung tissue, mouse liver tissue, mouse HEPA1-6 whole cell. IHC: human skin cancer tissue, mouse testis tissue, rat testis tissue, human ovary cancer tissue, human appendicitis tissue, human renal carcinoma tissue, human gastric cancer tissue, human melanoma tissue, human bladder cancer tissue, human tonsil tissue. ICC/IF: A431 cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          This gene encodes a member of the like-Sm family of proteins. The encoded protein consists of a closed barrel shape, made up of five anti-parallel beta strands and an alpha helix. This protein partners with six paralogs to form a heteroheptameric ring which transiently binds U6 small nuclear RNAs and is involved in the general maturation of RNA in the nucleus.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: LSM8 homolog, U6 small nuclear RNA associated; LSM8 U6 small nuclear RNA associated; MAK31-like protein; N-alpha-acetyltransferase 38, NatC auxiliary subunit; U6 snRNA-associated Sm-like protein LSm8; unnamed protein product

          View more View less

          Gene Aliases: 2010003I05Rik; AW214405; LSM8; NAA38

          View more View less

          UniProt ID: (Human) O95777, (Mouse) Q6ZWM4

          View more View less

          Entrez Gene ID: (Human) 51691, (Mouse) 76522, (Rat) 296913

          View more View less

          Function(s)
          RNA binding mRNA binding protein binding U6 snRNA binding poly(A) RNA binding U6 snRNA 3'-end binding RNA splicing factor
          Process(es)
          mRNA splicing, via spliceosome mRNA processing RNA splicing biological_process
          It has to be done as per old AB suggested Products section.

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