Immunofluorescent analysis of LXR alpha (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LXR alpha polyclonal antibody (Product # PA1-330) in 3% BSA-PBS at a dilution of 1:300 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C D(318) F S Y N R E D F A K A(329) of human LXR alpha.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||1 - 3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-330 detects recombinant human liver X receptor (LXR) alpha. This antibody does not detect human recombinant LXR beta protein.
PA1-330 has been successfully used in ICC/IF and Western blot procedures. By Western blot, this antibody detects an ~64 kDa protein representing recombinant human LXR alpha.
The PA1-330 immunogen is a synthetic peptide corresponding to residues C D(318) F S Y N R E D F A K A(329) of human LXR alpha. The PA1-330 immunizing peptide (Cat. # PEP-114) is available for use in neutralization and control experiments.
Liver X receptors (LXRs), also known as RLD1, are oxysterol activated nuclear receptors which are involved in the regulation of genesis and metabolism of cholesterols and bile acids. LXRs form heterodimeric complexes with the retinoic acid receptors (RARs) once activated by their respective oxysterol ligands. Two known isoforms, alpha and beta, are differentially expressed; the alpha isoform predominantly expressed in liver, whereas beta isoform expression has been shown to be ubiquitous.
The corresponding gene for the LXR alpha is NR1H3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Liver X receptor is a therapeutic target in collagen-induced arthritis.
PA1-330 was used in immunohistochemistry to demonstrate that liver X receptor inhibits the progression of arthritis symptoms in collagen-induced arthritis
|Chintalacharuvu SR,Sandusky GE,Burris TP,Burmer GC,Nagpal S||Arthritis and rheumatism (56:1365)||2007|
Endotoxin down-regulates ABCG5 and ABCG8 in mouse liver and ABCA1 and ABCG1 in J774 murine macrophages: differential role of LXR.
PA1-330 was used in western blot to investigate the role of liver X receptor (LXR) in the regulation of ATP binding cassette (ABC) transporters
|Khovidhunkit W,Moser AH,Shigenaga JK,Grunfeld C,Feingold KR||Journal of lipid research (44:1728)||2003|
Oxysterol-activated LXRalpha/RXR induces hSR-BI-promoter activity in hepatoma cells and preadipocytes.
PA1-330 was used in EMSA to investigate the regulatory effect of LXR on SR-BI expression in hepatoma cells and preadipocytes
|Malerød L,Juvet LK,Hanssen-Bauer A,Eskild W,Berg T||Biochemical and biophysical research communications (299:916)||2002|