Immunofluorescent analysis of LXR beta (green) showing positive staining in the nuclear envelope of C2C12 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an LXR polyclonal antibody (Product # PA1-333) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues R(113) Y A C R G S G T C Q M D A(126) of rat LXR beta.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-333 detects LXR beta in human, rat and mouse samples.
PA1-333 has been successfully used in Western blot and IF procedures. By Western blot, this antibody detects an ~51 kDa protein representing LXR beta in rat and mouse liver samples.
The PA1-333 immunogen is a synthetic peptide corresponding to residues R(113) Y A C R G S G T C Q M D A(126) of rat LXR beta. The PA1-333 immunizing peptide (Cat. # PEP-271) is available for use in neutralization and control experiments.
Liver X receptors (LXRs), also known as RLD1, are oxysterol activated nuclear receptors which are involved in the regulation of genesis and metabolism of cholesterols and bile acids. LXRs form heterodimeric complexes with the retinoic acid receptors (RARs) once activated by their respective oxysterol ligands. Two known isoforms, alpha and beta, are differentially expressed; the alpha isoform predominantly expressed in liver, whereas beta isoform expression has been shown to be ubiquitous.
The corresponding gene for the LXR beta is NR1H2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ALOX5AP Overexpression in Adipose Tissue Leads to LXA4 Production and Protection Against Diet-Induced Obesity and Insulin Resistance.
PA1-333 was used in western blot to characterize LXA4 production and protection against diet-induced obesity and insulin resistance due to Alox5ap overexpression in adipose tissue
|Elias I,Ferré T,Vilà L,Muñoz S,Casellas A,Garcia M,Molas M,Agudo J,Roca C,Ruberte J,Bosch F,Franckhauser S||Diabetes (65:2139)||2016|