|Tested species reactivity||Bovine|
|Published species reactivity||Bovine|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||PBS with 0.1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-40280 detects Lactoferrin from bovine samples.
PA1-40280 has been successfully used in immunoprecipitation, ELISA, and Western blot applications.
The PA1-40280 imunogen is bovine lactoferrin.
Lactoferrin is an approximately 80 kDa glycoprotein which was first isolated from milk and found in epithelia and most body fluids and secretions. Lactoferrin is secreted in plasma by neutrophils. Its plasma concentration represents a positive relation to the total pool of neutrophils and the rate of neutrophil turnover. In inflammation lactoferrin is released from secondary granules of neutrophilic leukocytes into the extracellular medium. Therefore the extracellular lactoferrin concentration can be used as an index for neutrophil activation.
Lactoferrin is able to strongly bind to iron and considered to have antibacterial properties. Human lactoferrin binds to bacterial products through its highly positively charged N terminus and kills various bacteria most probably by inducing intracellular changes in these bacteria without affecting the membrane permeability. Lactoferrin also plays a role in signal transduction, immunomodulation and has antiadhesive, anticancer, antiviral activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Lactoferrin and its hydrolysate bind directly to the oleate-activated form of the lipolysis stimulated lipoprotein receptor.
PA1-40280 was used in western blot to study the ability of the milk protein lactoferrin to bind to the free fatty acid activated form of the lipolysis-stimulated lipoprotein receptor and the significance for chylomicron clearance
|Ahmad N,Girardet JM,Akbar S,Lanhers MC,Paris C,Yen FT,Corbier C||The FEBS journal (279:4361)||2012|