Invitrogen

Latexin Polyclonal Antibody

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Latexin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using LXN (Product # PA5-28534) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Latexin Antibody in Western Blot (WB) — Western blot analysis of Latexin was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Latexin Polyclonal Antibody (Product # PA5-28534) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Latexin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — FIGURE 5 cFos levels increase in the claustrum, lateral hypothalamus (LHA), and locus coeruleus (LC) with 3 h sleep deprivation (SD) compared to sleeping control (SC) animals. (A) 3 h SC cFos staining; (A') 3 h sleep control with cFos and Latexin as marker for the claustrum; (B) 3 h SD cFos staining; (B') 3 h SD with cFos and Latexin; (C) 3 h SC cFos staining; (C') 3 h sleep control with cFos and Orexin as marker for the LHA; (D) 3 h SD cFos staining; (D') 3 h SD with cFos and Orexin; (E) 3 h SC cFos staining; (E') 3 h sleep control with cFos and tyrosine hydroxylase (TH) as marker for the LC; F) 3 h SD cFos staining; (F') 3 h SD with cFos and TH. Bar size A: 500 mum; C,E : 250 mum.

Product Details

PA5-28534

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 1 and 222 of Human LXN View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M tris glycine, pH 7, with 10% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2546010

Product Specific Information

Recommended positive controls: 293T, HeLaS3, Molt-4, mouse brain, rat brain, , rat heart.

Predicted reactivity: Mouse (85%), Rat (85%), Rhesus Monkey (97%), Bovine (85%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes the only known protein inhibitor of zinc-dependent metallocarboxypeptidases.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Acute Sleep Loss Upregulates the Synaptic Scaffolding Protein, Homer1a, in Non-canonical Sleep/Wake Brain Regions, Claustrum, Piriform and Cingulate Cortices.

Frontiers in neuroscience

Zhu J,Hafycz J,Keenan BT,Guo X,Pack A,Naidoo N

PA5-28534 was used in Immunocytochemistry, Immunohistochemistry to examined the expression of homer1a, an immediate early gene involved in intracellular signaling cascades, in mice subjected to extended wakefulness.